In marine macrobenthos studies the identification of organisms at species level is the best entry to ecological and biological information about the animals. An accurate identification requires excellent conservation of the organisms, reliable fauna description, experts and lengthy work in the laboratory. The aim of this work is to test taxonomic sufficiency (TS) in two deliberately selected different case studies to understand whether and how the taxonomic complexity of a benthic assemblage influences the results of TS and where it works better. The first benthic settlement was collected in an area characterized by homogeneous depth and grain size composition (case study A) around an off‐shore gas platform, while the second one was collected along a coast‐wide transect in an area with human pressure limited to fishing activities (case study B). Univariate and multivariate statistical analysis was used to assess differences in the taxonomic structure of benthic assemblages and to test TS on the two different datasets. TS seems to work in both sites, from species to higher taxonomic levels, and the family taxonomic level appears the best compromise for taxonomic resolution when an accurate identification is not achievable. The application of TS does not indicate a significant difference between the two datasets and appears therefore to be a valid instrument to analyse and describe the structure of benthic settlements in the case of taxonomically complex communities.

The aim of the study was to evaluate the long-term development of a hard bottom benthic assemblage over a period of 20 years in an area off the mouth of a large river. The artificial reef of Fregene was selected because benthic assemblage data were available for the period 1981-1992. This artificial reef is located in the mid Tyrrhenian Sea, 5 nautical miles north of the two mouths of the Tevere River (Latium, Italy) and 1.5 nautical miles offshore from Fregene (Rome, Italy). The artificial reef was deployed in March 1981 for fisheries enhancement in 10-14 m of water on a sandy-silty seabed. The Tevere River carries suspended materials and a heavy load of organics since it transports Rome's effluent, resulting in the eutrophic state of area waters. Benthic sampling was conducted in 2001 by SCUBA diving; two standard surfaces of 400 cm 2 were scraped from the vertical walls of the same uppermost block in four different periods. All organisms were identified and counted. The methodology used is the same as that adopted in the previous periods, so that the 2001 data could be compared with past collected data. The benthic assemblage was analysed by cluster analysis using the Bray-Curtis index and clustered using the group average clustering algorithm. The SIMPER procedure was used to identify those taxa that characterize each station group identified by cluster analysis. Changes in benthic assemblages and hydrological trends of the Tevere River were investigated using the cumulative sum series method. The 20-year development of the benthic community, starting from the new substratum, is composed of different phases characterised by different benthic assemblages. In particular five different phases were distinguished: 1. Pioneer species recruitment (May 1981-June 1981); 2. Mytilus galloprovincialis (mussel) dominance (August 1981-November 1983); 3. M. galloprovincialis regression (July 1984-October 1985); 4. M. galloprovincialis absence (91-92); 5. Bryozoans bioconstruction dominance (2001). The dynamic succession of the observed benthic assemblages exhibited a good relation with the Tevere River flow. The Tevere River flow, and the subsequent sedimentation process, seems to have strongly influenced the benthic assemblage succession of the Fregene artificial reef

The aim of the study was to evaluate the long-term development of a hard bottom benthic assemblage over a period of 20 years in an area off the mouth of a large river. The artificial reef of Fregene was selected because benthic assemblage data were available for the period 1981-1992. This artificial reef is located in the mid Tyrrhenian Sea, 5 nautical miles north of the two mouths of the Tevere River (Latium, Italy) and 1.5 nautical miles offshore from Fregene (Rome, Italy). The artificial reef was deployed in March 1981 for fisheries enhancement in 10-14 m of water on a sandy-silty seabed. The Tevere River carries suspended materials and a heavy load of organics since it transports Rome's effluent, resulting in the eutrophic state of area waters. Benthic sampling was conducted in 2001 by SCUBA diving; two standard surfaces of 400 cm 2 were scraped from the vertical walls of the same uppermost block in four different periods. All organisms were identified and counted. The methodology used is the same as that adopted in the previous periods, so that the 2001 data could be compared with past collected data. The benthic assemblage was analysed by cluster analysis using the Bray-Curtis index and clustered using the group average clustering algorithm. The SIMPER procedure was used to identify those taxa that characterize each station group identified by cluster analysis. Changes in benthic assemblages and hydrological trends of the Tevere River were investigated using the cumulative sum series method. The 20-year development of the benthic community, starting from the new substratum, is composed of different phases characterised by different benthic assemblages. In particular five different phases were distinguished: 1. Pioneer species recruitment (May 1981-June 1981); 2. Mytilus galloprovincialis (mussel) dominance (August 1981-November 1983; 3. M. galloprovincialis regression (July 1984-October 1985; 4. M. galloprovincialis absence (91-92); 5. Bryozoans bioconstruction dominance (2001). The dynamic succession of the observed benthic assemblages exhibited a good relation with the Tevere River flow. The Tevere River flow, and the subsequent sedimentation process, seems to have strongly influenced the benthic assemblage succession of the Fregene artificial reef.

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