SE~RZAN, P., CLIAIJ-WOM;, M., and MOYYFN, S. 8972. The r-nicnabranc binding of morphine, diphenylhydantoin, and teirahydrocannabi~aol. Can. J . Physiol. Pharrnasol. 50, 1193-1200.The adsorption of rnorgshirae to guinea pig brain synaptosornc membranes and to human erythrocyte rncmbranes was found lo be passive, and unaffectec8 by time, tanperature, o~aabain, dinitrophenol, saponin, or ATP. ' Y' he mernbrane/buffer partition coefficient for niorphine was 35 (at low ionic strength) and i . 2 (at high ionic strength). The synaptosome membrane/buffer partition coefficient for diphenylk~ydaralclin was around 60 (at pH 8), while that for A()-tetrahydrocannabinol (THC) was around 380 (in the concentration range of around 10-"M). The partition cc>efficient for the latter dsug dropped by a factor of two G;. three with increasing drug col-mcentrations for both erythrocyte ghosts and synaptosomes; there may be two types of binding sitcs for THC. The minimu111Can. J. Physiol. Pharmacol. Downloaded from www.nrcresearchpress.com by University of Auckland on 12/07/14For personal use only.Fnc;. 4. A double reciprocal plot of thc data of Fig. 3 indicates that the points fall on a single line, suggesting that there is one set of binding sites h r diphenylhydantoin (at p H 10.5).Can. J. Physiol. Pharmacol. Downloaded from www.nrcresearchpress.com by University of Auckland on 12/07/14For personal use only.