Wound healing is a dynamic phenomenon that results in the restoration of anatomic continuity and function on which can be delayed by pathogenic bacteria. This research was designed to explore the antimicrobial efficacy of Tillandsia usneoides against skin infections in wound healing. Physical and chemical evaluations were done through phytochemical screening and thin layer chromatography. The effect of methanolic, ethanolic and aqueous extracts of Tillandsia usneoides against pathogenic bacteria were evaluated on antimicrobial activities using disc diffusion and broth dilution susceptibility assay. The excision bioassay analysis was used in examining the wound healing process in mice. The result of the qualitative phytochemical screening showed the presence of flavonoids and alkaloids. Thin layer chromatography revealed a high Rf value for flavonoids (0.75mm) and alkaloids (0.60mm). The antibacterial assay showed a high zone of inhibition (ZI) for both methanolic extract (>23mm, >22mm and >20mm) and ethanolic extract (>22mm, >22mm and >17mm) for P.aeruginosa, S.aureus and S.epidermis respectively. Resistance against aqueous extract was observed based on the lowest zone of inhibition ( less than 4mm). Gentamicin was used as the positive control (>28mm) and DMSO as the negative control. The minimum inhibitory concentration and minimum bactericidal concentration results confirmed that methanolic and ethanolic extracts restrained the growth of tested bacteria in the range of 125 to 500mg/mL and showed bactericidal efficacy. Wound healing assay indicated that methanolic extract had a higher potency of wound closure (12 days; less than 1mm) compared to ethanolic and aqueous extracts (13days; >1.5mm and 13days: >1.8mm respectively). Povidone-Iodine was used as the gold standard (15days; less than 2mm) in the study. In conclusion, methanolic extract of T. usneoides has a great potential with regard to its antimicrobial and wound healing activity to be developed as a novel drug in the future.