The 100 kV bushing is one of the most important and technologically challenging Safety Important Class (SIC) components of the Diagnostic Neutral Beam (DNB) injector of ITER. It forms interface between gas insulated electrical transmission line and torus primary vacuum and acts as a vacuum feedthrough of ITER. Design optimization has been carried out to meet the electric and structural requirements based on its classification. Unlike HNB bushing, single stage bushing is designed to provide 100 kV isolation. Finite Element Analysis (FEA) based optimization has been carried out for electrostatic and structural analysis. Manufacturing assembly sequence is studied and presented in this paper. However validation of the same is foreseen from manufacturer.