The increasing resistance of Plasmodium falciparum to available antimalarial drugs currently justifies the quest for potential antiplasmodial agents from plants. Picralima nitida is used as a traditional remedy against malaria in Nigeria. In this study, phytochemical constituents of methanolic and aqueous extracts of P. nitida fruit pulp were screened, and tested in vitro against 20 clinical isolates of P. falciparum using the schizont growth inhibition assay. Acute oral toxicity of each extract was also evaluated in white albino mice. The results showed the presence of alkaloids, anthraquinones, flavonoids, saponins, and tannins in both extracts. Terpenes were only present in the methanolic extract. In vitro growth inhibition of P. falciparum by each extract was dose dependent. The methanolic extract exhibited moderate, but higher antimalarial activity with IC 50 = 23.33 ± 0.76 µg/ml, compared to the aqueous extract with IC 50 = 28.25 ± 0.95 µg/ml. The difference was significant (t = 16.72, df = 19, P < 0.001). The LD 50 of each extract was 3807 mg/kg body weight. The moderate antiplasmodial, and toxicity of these extracts, may justify the use of P. nitida, fruit pulp for the treatment of malaria in Nigeria.