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Eight strains of Bacillus spp. were examined for production of toxin (s). Three strains of Bacillus cereus produced toxins lethal to mice. The culture Jiltrate of Bacillus cereus BIS-59 contained two lethal toxins. One was a hemolysin and the other a nonhemolytic glycoprotein. The growth of cells in fresh beef infusion broth resulted in high toxin production, compared to growth in tryptone-glucoseyeast extract or nutrient broth. Separate identities for the two toxins from B .vcereus BIS-59 were established on the basis of hemolytic activity, separation pro$les on Sephadex gel $filtration, responses to heat and radiation and kinetics of their production during the growth of the organism.

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