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This report describes the effects of therapeutic doses of coccidiocid sulphachloropyrazine on enzymatic and non-enzymatic antioxidative systems in haemolysed blood and liver homogenate from broilers (glutathione, glutathione-reductase, glutathione-peroxidase, peroxidase, superoxidedismutase, xantine-oxidase and lipid peroxidation). The in vivo investigation was carried out on 120 heavy-line broilers (Arbor acres) of both sexes. One-day-old broilers were randomly distributed into 2 groups, each numbering 60 individuals of both sexes: Group 1 -control group; Group 2 -group of broilers inoculated with laboratory derived coccidia species on the 21 st day-ofage. When symptoms of coccidiosis appeared (30 th day-of-age), blood sampling and decapitation of 20 chickens were carried out (Group 2a). The remaining broilers were treated with therapeutic doses of sulphachloropyrazine (60 ppm). Decapitation of 20 chickens was carried out after the therapy was concluded (38 th day-of-age -Group 2b). Infection of broilers with coccidia intensified free radical processing in haemolysed blood and liver homogenate. This was evident from the increased levels of lipid peroxidation and the catalytic activity of almost all examined enzymes (SOD, GSHPx and Px). Therapeutic doses of sulphachloropyrazine inhibited free-radical activity induced by disease and establishing of physiological values of lipid peroxidation and catalase activity of examined enzymes.

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Long term pollution of selected aquatic resources of Vojvodina with polychlorinated biphenyls (PCB's) and organochlorine pesticides (OCP's) has led to high degree of bioaccumulation of the above chemicals along the food chain. This paper documents the bioaccumulation in two fish species commonly found in rivers and canals of Vojvodina, common carp (Cyprinus Carpio) and pike (Exos lucinus). Samples offish, sediment and water were collected from the Danube, Sava and Tisa rivers and a fish farm “Ečka” which is supplied with water from the river Tisa and river Begej. All samples were analysed by highly sensitive capillary column GC using EC detectors. Samples were analysedfor aspectrum of compounds and are presented here as total PCB's and OCP's. The results show that the studied compounds are present in water samples at very low concentrations (PCB's in concentrations at or below the limit of detection, OCP's in concentrations below the legally allowable concentrations by Yugoslav norms). The sediment samples contain the studied compounds at significantly higher concentrations but still within the expectable range. Fish tissue contained elevated concentrations, and especially so the tissue of the top predator in the food chain, the Pike, showing that bioaccumulation has occurred. Of special concern are the extremely high concentrations of PCB's in the tissue of fish samples collected from river Sava indicating the still existing effects of pollution by PCB's in the watershed of the river Sava.

In this paper, the concentration of a wide range of persistent organochlo-rine compounds α, β,γ, hexachlorocyclohexane Ʃ-HCH, p,p 'DDE, o,p 'DDE, p,p 'DDT and polychlorinated biphenyls (PCBs) in the aquatic environments of the river Danube, Tisa, Lake Palić, and fish-pond Ečka are reported. The residues of organochlorine insecticides -OCI and PCBs in water, sediments, fish muscle and eggs of aquatic birds were analysed. These data show the serious contamination of selected aquatic ecosystems (Danube, Tisa, Lake Palić and fish-pond Ečka) of Vojvodina. Because of the concentration of these compounds at top levels, fish, eggs of aquatic birds and sediments are an excellent indicator for detecting trace organic compounds whose presence in water would be difficult to measure directly. The residues of OCI were determined by GC-ECD. Polychlorinated biphenyls -- PCB congeners were quantified by high-resolution gas chromatography (HRGC-ECD).