Abstract-Fluoride-releasing materials are commonly used to restore cervical lesions and in reconstructive periodontal surgery. The attachment and proliferation of various materials on human periodontal ligament fibroblast (HPDL) cells were evaluated in this study. Four fluoride-releasing restorative materials were tested: GIC (F9), RMGIC (F2), Giomer (B), and resin composite (G); while resin composite (P) was used as a negative control. The specimens were prepared according to the standard protocol, and then primary cultures of HPDL cells were seeded on specimens and control glass cover slips. The attached cells were counted at 1, 3, 24, and 72 h after cell seeding. Cell proliferation was determined using MTT assay at 1, 3, 5, and 7 days after cell seeding. The cell morphology was determined by SEM. Cell attachment increased as time elapsed for all materials. After initial attachment, Phad the best cell attachment profile when compared to the other groups. G had the greatest amount of cell attachment at the end of cell culture time, while F9 showed the least. The cell proliferation profile of G was the highest, while F9 and F2 were the least (p < 0.05) among all groups. SEM evaluation determined that HPDL cells on the materials were round or oval at the initial time of cell culture. As time elapsed, HPDL cells presented a variety of cell morphologies in the development of cytoplasmic processes.

75:25 poly (DL-lactide-co-ε-caprolactone) electrospun resorbable membranes were prepared by electrospinning and film casting techniques. Primary cultures of human periodontal ligament (HPDL) fibroblasts cells were seeded on the electrospun membranes, film-cast membranes, and control glass cover slips. The number of cell attached was determined at 1, 3, 24 and 72 h after cell seeding. Cell proliferation on the membranes was determined by MTT assay at 1.5 h and 1, 3, 5 and 7 days after cell seeding. The morphology of cells was also determined by SEM. The results indicated that the cell attached number and cell proliferation on the electrospun membranes were significantly higher than that of the film-cast membranes at every time point. From an SEM study, HPDL cells could healthy attach on both, the electrospun membranes and the film-cast membranes. HPDL cells were firmly attached to the membrane matrix in the electrospun membrane group. In the film-cast group, HPDL were loosely attached to the membrane surfaces. In conclusion, an electrospun membrane had better ability to promote HPDL cell attachment and proliferation than did film-cast membranes.