The myostatin gene of seven important meat (Beltex (Australia), Beltex×Huyang (F1), Meat and Multi-Prolific Chinese Merino Fine Wool, Meat Chinese Merino Fine Wool and Dorper (South Africa)) and non-meat (Huyang and Kazak) sheep breeds was analyzed to study the genetic basis of muscular hypertrophy (double muscling) phenotype in sheep. SNPs, four in regulatory regions and several in the introns in the myostatin gene, were identified, and the former four SNPs were used for further studies. Twelve haplotypes were predicted by PHASE program, of which four main haplotypes (1, 3, 7, 9) were present in 90% of the 364 sheep in the study. Haplotypes 1-4 were mainly present in meat breeds while haplotypes 7 and 9 dominated the non-meat breeds. The association between haplotypes and average daily gain (ADG) was analyzed among 116 sheep with production data, Haplo2 (CGAA) and Haplo8 (TGAA) were identified to have significant (p<0.05) effect on ADG by the model (JMP5.1 software) taking into account the effects of breed, family background, haplotype, birth weight and sex. ADG of these haplotype groups also correlated well (r = 0.82) with hypertrophic phenotype scores. In conclusion, the mutations -956 (T→C), -41 (C→A) and 6223 (G→A) involved in Haplo2 and 8 may be associated with the double-muscling trait by influencing myostatin function and be suitable markers in selecting meat sheep.
In this paper, we successfully fabricate compact and uniform cadmium telluride (CdTe) films on flexible nickel foils using simple electrodeposition technique and sintering in a nitrogen atmosphere. The effect of the concentration of sodium tartrate on the structures and properties of the deposited CdTe thin films are studied. Various test techniques are used to characterize the results. The results of XRD analysis show that the highest crystallinity film is obtained when the added sodium tartrate concentration is 0.05 M. With the increase of sodium tartrate concentration, the composition of the film gradually changes from Te-rich to Cd-rich, and the thickness of the film becomes thin gradually. We also find that the CdTe thin film prepared under 0.05 M sodium tartrate shows the best optical and optoelectronic properties. Thus, a moderate amount of sodium tartrate is added into an acidic solution as surfactant for CdTe thin film fabrication. Moreover, sodium tartrate is easily volatilized from the film during the annealing process because of its small molecular weight, which can prevent the carbon precipitation.
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