A rapid and efficient protocol was developed for inducing indirect organogenesis using leaf explants of Vitex negundo L. Explants were cultured on MS with different concentrations of 2,4-D and IAA in combination with BAP for callus induction. The frequency of callus induction increased with increasing concentration of IAA (0.3 mg/l) and BAP (0.3 mg/l) at optimal level. The shoot buds appeared emerging as green coloured protuberances on the callus. The high frequency of shoot bud initiation and shoot proliferation was observed on MS containing 0.3 mg/l IAA and 0.3 mg/l BAP. The regenerated shoots were successfully rooted on MS supplemented with 0.5 mg/l IBA. Rooted plants were transferred to pots containing sand, soil and manure in the ratio of 1 : 1 : 1. Nearly 90% survival of in vitro plants were recorded.

A method for induction of multiple shoots using axillary bud and shoot tips of Sphaeranthus amaranthoides Burm. f. was described. The experiment was conducted in which multiple shoot induction was noticed on MS (Murashige and Skoog) medium supplemented with different concentrations of two cytokinins (BAP and Kin). These multiple shoots later developed into normal shoots. The highest rate of shoot proliferation was found to be 90% and 75% for axillary bud and shoot tip explants, respectively, on the medium containing BAP 4.0 mg/l. The regenerated shoots were successfully rooted on MS medium supplemented with 2.0 mg/l IBA, after sequential hardening, survival rate was 90%.