We estimated the effect on growth and nutrient efficiency of replacing fishmeal with silage incorporated with rice bran in diets for fingerling red tilapia ( Oreochromis mossambicus × Oreochromis niloticus × Oreochromis aureus) over 12 weeks. Isonitrogenous (300 g kg −1 protein dry matter basis) and isoenergetic (4450 Kcal gross energy kg −1 ) feed formulations with increasing levels of tilapia silage as a replacement for fishmeal were prepared: Diet 1 with no silage (0 g Kg −1 ), Diet 2 (250 g Kg −1 ), Diet 3 (500 g Kg −1 ), and Diet 4 (750 g Kg −1 ). Feed intake was similar among Diets 1, 2, and 3, while Diet 4 had a significantly lower intake. There was no significant difference (P > 0.05) in weight gain or specific growth rate (SGR), feed conversion ratio (FCR), and protein efficiency ratio (PER), among fish fed Diets 1, 2, and 3. Fish fed with Diet 4 had significantly lower weight gain; SGR and PER and significantly higher FCR. Organoleptic properties of the fish were not affected by the diets. The results of this study indicate that less expensive dried fish silage with rice bran is an alternative protein source for tilapia feed up to 50% of fishmeal replacement.

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The most prevalent malnutrition issues in Sri Lanka are the protein-energy malnutrition (PEM), vitamin A deficiency, and iron deficiency. Childhood and adolescence are the crucial stages of life which mostly susceptible to malnutrition since their major physiological and psychological changes take place. Although a balanced, nutritious diet is essential to them, present socio-economical and higher academic expectations have created a complex environment that often pushes them towards junk foods and unhealthy snacks. The objective of the present study was to develop ready-to-reconstitute convenient mid-day meals in three categories of chicken, fish, and soya, which match to the Sri Lankan local culinary style, with acceptable sensory properties to upgrade the nutritional status of school children (5-10 yrs) and adolescents (11-19 yrs). Those mid-day meals were designed according to the Food-Based Dietary Guidelines (FBDG) of Sri Lanka. The selected ingredients were processed; rice to instant rice, vegetables to dehydrated vegetables, and protein source of chicken, fish, and soya to pre-cooked sterilized curry pouches. The convenient mid-day meal packages were prepared using three portions of instant rice containing 10% of green gram flakes, dehydrated vegetables, and retorted curry mix respectively. Labeling was performed according to the type of curry mix inserted into the package as Chicken, Fish, or Soy mid-day meals. Physicochemical and sensorial properties of meals were evaluated. The caloric value of mid-day meals ranged 575 - 640.82 kcal. The protein content of meals ranged from 25.88 g to 32.68g and there was no significant difference (p<0.05) between protein contents in chicken and fish meals. The fat contents and dietary fiber contents significantly (p<0.05) differed and the highest contents of fat (3.15 g) and dietary fiber (4.40 g) were observed in chicken meal and soy meal respectively. The mineral contents of tested meals significantly differed (p<0.05) and the significant highest Ca, Fe, K, Fe, and P were observed in soya mid–day meals. The highest mean rank scores for all sensory attributes in terms of odor, appearance, texture, taste, and overall acceptability were observed in the chicken mid-day meal. Ready-to-reconstitute mid-day meals in three categories of chicken, fish, and soya, which could match to the Sri Lankan local culinary style, were developed with acceptable sensory properties.

Aims: The objective of this study was to investigate on microbial related food safety issues and microorganisms associated with the production of Sri Lankan traditional seafood Jaadi. Place and Duration of Study: Food Technology Section, Industrial Technology Institute, Colombo, Sri Lanka, between December 2013 to April 2016. Methodology: Seven Jaadi processing centers along the Southern and Western coastal belt of Sri Lanka were evaluated for its compliance to food safety, in terms of environment, processing techniques, hygiene and sanitation, physiochemical and microbiological quality of the final product.  Samples drawn from all sites were analyzed for its water activity, salt content, pH and microbiological quality.  Microorganisms associated with Jaadi production were isolated in selective media, followed by phenotypical, biochemical and molecular biological characterization. Results: The pH, water activity and salt content of Jaadi samples ranged between 3.60-5.85, 0.73-0.82 and 24.82-40.47%, respectively. Thirteen bacterial strains and one fungal strain detected were strains found to be responsible for human pathogenesis and food spoilage. These include,  Bacillus cereus strain I (MN726935.1), Bacillus cereus strain II (MN901259.1), Bacillus haikouensis strain I (MN901262.1), Bacillus haikouensis strain II (MN726976.1), Bacillus licheniformis (MN726987.1), Acinetobacter baumannii (MN901499.1), Bacillus pumilus strain I (MN901264.1), Bacillus pumilus  strain II (MN901263.1), Bacillus paralicheniformis (MN901167.1), Bacillus thuringiensis strain I (MN901165.1), Bacillus thuringiensis strain II (MN901257.1), Bacillus cereus (MN901161.1), Staphylococcus saprophyticus (MN901156.1) and Trichoderma longibrachiatum (MN907169.1).  Presence of such organisms clearly proclaims the poor hygienic practices and risks related to food safety in this traditional processing technology. Conclusion: Jaadi processing facilities of Southern and Western coastal belt of Sri Lanka needs scientific and technical knowledge to upgrade their processing in order to assure food safety and product quality.

King coconut water (KCW) is a sweet relish product that is more prone to rapid quality deterioration, and several safety concerns are emerging due to its inappropriate thermal processing. Therefore, the objective of this study was to identify the potential spoilage/pathogenic microorganisms associated with the processing of KCW, with the assessment of possible physicochemical changes as providing preliminary information required for the thermal process validation of bottled KCW. Samples (n = 6, 150 ml/sample) were collected from three different KCW processing facilities at five critical processing steps P 1 − P 5 . A facility survey, physicochemical analyses, and microbial enumeration and isolation, along with their molecular identifications, were conducted. It was found that all tested physicochemical properties were significantly changed p < 0.05 among sampling points at each processing facility. The colour of thermally processed KCW samples has significantly changed p < 0.05 compared to the fresh KCW, which causes a distinct effect on the appealing quality of the final product. A pattern of initial lower counts with gradually increased microbial counts at intermediate processing steps (1.0 × 103–5.3 × 106 CFU/ml) and significantly lowered p < 0.05 counts after thermal treatment was observed. Among the bacterial and fungal isolates identified, several potential pathogenic bacterial species, such as Pantoea dispersa, Bacillus siamensis, Pseudomonas stutzeri, and Acinetobacter lactucae; a few thermal resistant yeasts, Pichia kudriavzevii, Debaryomyces nepalensis, and Candida carpophila; and moulds, Penicillium citrinum, Microdochium fisheri, and Trichosporon asahii, have survived in the thermally processed KCW. Based on the results of the study, it is suggested that the thermal process validation of KCW should be targeted according to the revealed knowledge on the identified hazardous microorganisms, while adhering to Good Manufacturing and Hygienic Practices with minimized handling time to avoid rapid quality deterioration.