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Aim:The aim of our work was to compare the de-epithelialization graft method with the standard single incision technique for harvesting of connective tissue grafts. The viability potential of the graft was examined histologically & immunohistochemically and histomorphometrically.Methodology: New Zealand rabbits were divided into 2 groups: single incision group (10 rabbits) lancet 15c was used to perform a pouch for harvesting the connective tissue graft, and De-epithelialization group (10 rabbits), where de-epithelization was performed using flat stone and the dimensions of the donor area were measured using a millimeter periodontal probe 3 mm in width and 10 mm in length. The harvested connective tissue graft was examined histologically and immunohistochemically for CK10 expression. The graft was analyzed by flow cytometry for necrotic cells assay. Hitsomorphometric evaluation of CK10 positive surface area was done. Results:The study demonstrated that signficant decrease was detected in a number of necrotic cells in the de-epithelialization group as compared to the single incision group. CK10 positively reacted epithelial remnants were more in the de-epithelialization group than the single incision group, but this increase was non-significant. Conclusion:The de-epithelialization grafting technique was found to be a simple procedure.Additionally, it showed large areas of extravasated red blood cells and more adipose tissues were obvious in the single incision group as compared to the de-epithelialization group with less technical complication during the surgery. Furthermore, it revealed less necrotic cells, less hemorrhage, and more accurate in the separation of epithelium from the connective tissue.

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