Background: This particular study emphasis on the in vivo wound healing and in vitro antibacterial activity of herbal lotions preparated from Ethanolic extract of Justicia tranquebariensis, Aloe vera and Curcuma longa. Methods: Each plant powder (10 g) was soaked in 30 ml of ethanol in a sterile bottle for 48 h at 4°C. Herbal lotion formulation I (10%) and II (20%) was prepared. Antibacterial activity by Agar Well diffusion method for three medicinal plant lotions was tested against skin and wound pathogens, like Staphylococcus aureus, Bacillus subtilis, Escherichia coli and Klebsiella pneumoniae. The excision wound model was employed for wound healing activity in albino rats. Healthy albino rats (150-200 g) of either sex were taken for excision wound model. Animals were divided into four groups of six animals in each. The Group 1 is control. The Groups 2 and 3 animals were treated with 10% and 20% ointment of herbal lotion and Group 4 animals were treated with Soframycin that served as standard. All animals had free access to pelleted food and water. Temperature was maintained at 23 ± 1°C. The results were expressed as mean ± SEM. Further sample tissues were fixed in 10% buffered formalin, processed and blocked with paraffin at 40-60°C, and then sectioned into 5 μm thick sections. The significance of differences between the means was analyzed by student's t-test followed by Turkey's test. Results: Complete wound healing was observed with lotion formulation I and II treated rats in 15 days as that of Soframycin ointment. These findings were further confirmed by histological examination of granulation tissue with a lesser amount of collagination and absence of inflammatory cells. Conclusion: The findings from this research indicates that the ethanol extract of Justicia tranquebariensis, Aloe vera and Curcuma longa are effective in inhibiting the growth of wound associated pathogen and faster the process of wound healing.

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Abstract. There are thousands of protected forest areas existing on earth, yet the deforestation rate continues unabated both inside and outside the protected areas especially in the tropical forests. It identifies the less effectiveness of the current conservation strategies, which is normally oriented around the forest area cover rather than the quality of the protected areas. This calls for realistic and effective management strategies for forests. Based on the drawbacks the present study aims at identifying conservation priority sites within the protected areas (Reserved Forests) of Shervarayan hills, Eastern Ghats of Tamil Nadu, India. The remnant forest patches having less effective management/protection is identified and analysed for its qualitative contribution to the ecosystem. Quadrats of 20 · 20 m were laid in different vegetation based on the percentage of forest cover and assess the species diversity pertaining the richness, Endemism and Red list categories. Thematic layers (maps) such as vegetation type, floristic species richness, floristic endemism, and red list flora are created and categorized according to their weightage classes and overlaid in GIS domain to demarcate the Conservation Priority Zones (CPZ). The CPZ are classified according to the priority status i.e., high, moderate and low based on the contributing species richness, levels of endemism and concentration of Red listed plants.

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sequestration potential under existing agroforestry systems for any district or region.