The effect of the intravenous injection of 100 mcg (2 ml) gonadotropin-releasing hormone (Ovarelin, Ceva) from the 1st to the 20th day after calving of beef cows was monitored in the article, in regard to the occurrence of the first oestrus and the possibilities for its controlling. It was established that in the first minutes of intravenous application of Ovarelin the concentration of luteinizing hormone (LH) increased. By increasing the number of days increased the amount of secreted LH. Maximum effect of the injection of 100 mcg GnRH occurred between the 10th and 15th day. The physiological dose (100 mcg) GnRH injected intravenously on the 1st day after birth of cows led to increased content of LH in blood circulation.

Mesenchymal stem cells (MSCs) are involved in the process of extracellular matrix (ECM) remodeling where collagens play a pivotal role. We recently demonstrated that the remodeling of adsorbed collagen type I might be disordered upon oxidation following its fate in the presence of human adipose-derived MSC (ADMSCs). With the present study we intended to learn more about the effect of polyphenolic antioxidant Epigallocatechin-3-gallate (EGCG), attempting to mimic the conditions of oxidative stress in vivo and its putative prevention by antioxidants. Collagen Type I was isolated from mouse tail tendon (MTC) and labelled with FITC before being oxidized according to Fe2+/H2O2 protocol. FITC-collagen remodeling by ADMSC was assessed morphologically before and after EGCG pretreatment and confirmed via detailed morphometric analysis measuring the anisotropy index (AI) and fluorescence intensity (FI) in selected regions of interest (ROI), namely: outside the cells, over the cells, and central (nuclear/perinuclear) region, whereas the pericellular proteolytic activity was measured by de-quenching fluorescent collagen probes (FRET effect). Here we provide morphological evidence that MTC undergoes significant reorganization by the adhering ADMSC and is accompanied by a substantial activation of pericellular proteolysis, and further confirm that both processes are suppressed upon collagen oxidation. An important observation was that this abrogated remodeling cannot be prevented by the EGCG pretreatment. Conversely, the detailed morphometric analysis showed that oxidized FITC-collagen tends to accumulate beneath cells and around cell nuclei, suggesting the activation of alternative routes for its removal, such as internalization and/or transcytosis. Morphometric analysis also revealed that both processes are supported by EGCG pretreatment.

Different schemes of PMSG treatment on some reproductive parameters in sheep were studied. Two groups of 6 sheep each at the end of the non breeding season were treated with vaginal sponges Chrono-gest (Intervet, Holland) containing 30 mg fluorogeston acetate (FGA). The sponges were removed 12 days after insertion. Sheep of the experimental group were injected with 500UI Folligon (Intervet, Holland) 48 hours before sponge removal whilst the corresponding control sheep were treated with the same dose Folligon at the day of of sponge withdrawal. The clinical signs of oestrus, plasma progesterone concentrations and changes in the ovarian morphology during the observation period were registered. The conception rate, fertility and twinning rate were also recorded. The results show that the earlier application of the gonadotropin influence positively the reproductive traits in ewes.

Mesenchymal stem cells (MSCs) are involved in the process of extracellular matrix (ECM) remodeling where collagens play a pivotal role. We recently demonstrated that the remodeling of adsorbed collagen type I might be disordered upon oxidation following its fate in the presence of human adipose-derived MSC (ADMSCs). With the present study, we intended to learn more about the effect of polyphenolic antioxidant Epigallocatechin gallate (EGCG) attempting to mimic the conditions of oxidative stress in vivo and its putative prevention by antioxidants. Collagen Type I was isolated from mouse tail tendon (MTC) and labeled with FITC before oxidizing according to Fe2+/H2O2 protocol. FITC-collagen remodeling by ADMSC was assessed morphologically before and after EGCG pretreatment and confirmed via detailed morphometry analysis measuring the anisotropy index (AI) and fluorescence intensity (FI) in selected regions of interest (ROI), namely: outside the cells; over the cells and central (nuclear perinuclear) region, whereas the pericellular proteolytic activity was measured by de-quenching of fluorescent collagen probes (FRET effect). Here we provide morphological evidence that MTC undergoes significant reorganization by the adhering ADMSC along with the substantial activation of pericellular proteolysis, and further confirm that both processes are suppressed upon collagen oxidation. An important observation was that this abrogated remodeling cannot be prevented by the EGCG pretreatment. Conversely, the detailed morphometry analysis showed that oxidized FITC-collagen rather tends to accumulate beneath the cells and around the cell’s nuclei suggesting the activation of alternative routes for its removal, such as internalization and/or transcytosis. Morphometry analysis also revealed that both processes are supported by EGCG pretreatment.

The article evaluates the development of the Internet of Things (I₀T), digital technologies, various types of biological and biometric sensors and blockchain technologies in dairy and beef cattle breeding. The peculiarities, tendencies and perspectives for digital transformation and digitalization of the cattle farms and complexes have been studied.Precise technologies (PFL) make it possible to collect a sufficient cloud of data in accordance with the physiological and technological requirements of the various categories of animals of the species Bos taurus and the welfare of cattle.Biological and biometric sensors help farmers to increase the quantity and improve the quality of their products. Blockchain technologies present cattle breeding in detail, as transparent, stable and predictable in the eyes of the consumer. Cattle breeding is a sub-sector of animal husbandry in which there is no integration, but flexible digital management is applied.