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Acetate-1-14C was added to the culture medium of different species of mycobacteria in the exponential phase of growth. Analyses after incubation for 2 hours showed that acetate was utilized for the synthesis of various lipid fractions, and also converted into carbon dioxide. Mycobacterium tuberculosis strains, in contrast to saprophytic mycobacteria, showed preferential utilization of acetate for oxidation over utilization for lipid synthesis. In the virulent strain of M. tuberculosis a greater proportion of the total radioactivity in lipids was present in the non-saponifiable fraction than in other mycobacteria, including M. tuberculosis H37Ra.

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