The human peripheral blood lymphocyte provides a readily available in vitro system, representative of the donor's immune status.1 This cell responds to specific antigens to which the donor is sensitized, as well as to certain nonspecific stimulants, such as phytohemagglutinin (PHA), an extract of the kidney bean, Phaseolus vulgaris. PHA has been used for the past 5 years as a mitogenic agent for lympho

ExtractThis paper reports the cytogenetic investigation of nine children exposed to LSD-25 ,in utero, four children of'other LSD users not exposed in utero, the mothers of these children, and six additional adults taking the drug. In comparison with matched controls, significantly elevated frequencies of chromosomal damage were observed in all the individuals exposed. The frequencies of aberrations, however, were markedly reduced in those children not exposed in utero. Eight structural rearrangements were observed, six dicentric chromosomes and two quadriradial formations, among those individuals exposed to the drug, but none was observed in the controls. In spite of obvious chromosomal aberrations, all of the individuals in this study were apparently healthy and showed no obvious birth defects. Speculation