Ayurveda which is the science of life is famous all over the world because of its unique concepts and practising methods. The main aim of Ayurveda is to attain Dhatusamyakriya, and through that maintain the Swasthyata of the body. Anupana is one of the unique concept of Ayurveda which plays an important role in the treatment. Anupana is that material which is consumed along with food or medicine. It can increase the palatability of the food or medicine, can improve the digestion and absorption and also act as a vehicle which carries the drug to their target site. Hence this article deals with the importance of Anupana in the Ayurvedic practise.
Xylanase producing thermophilic actinomycetes strain B42 was isolated from bagasse. This strain was enriched on oat spelt xylan agar medium and screened onto xylan-congo red agar plate by the xylanolysis method. The Phylogenetic analysis using 16S rDNA sequence data showed that strain B42 had the highest homology (99.0%) with Laceyella sacchari and it was named as L. sacchari strain B42. L. sacchari strain B42 xylanase was purified to study its biochemical characteristics and its biobleaching efficiency. The partial purification of xylanase using acetone fractionation (at 1:3.0 ratio) gave 2.51 fold purification and the recovery of 88%. Further purification of the partially purified xylanase using DEAE-Sephadex A-50 and G-100 column chromatography gave 11.41 fold purification and 22.80% yield with the specific activity of 1750.0 U/mg. The molecular mass of the purified xylanase was ~30.0 kDa, as analyzed by SDS/PAGE and zymogram. The enzyme reactions followed Michaelis-Menten kinetics with Km and Vmax values of 4.166 mM and 3787.87µmole/min/ml/mg, respectively, as obtained from a Lineweaver-Burk plot. The optimal temperature of the enzyme was 70°C. The enzyme retained 72% of its activity at 70°C and 48% activity at 80°C after 6 h of incubation. The half life (t 1/2 ) of purified xylanase was 6 h at 80°C. The optimal pH of xylanase activity was 10.0 and enzyme appeared to be stable over a broad pH range (pH, 11.0 to 12.0) under the assay conditions. Approximately 68 and 64% of the original activity was retained after 5 h of incubation at pH, 10.0 and 11.0, respectively. The enzymatic biobleaching of kraft pulp reduced ~26% kappa number, decreased 1.68% lignin content and released 24 fold reducing sugars. The enzyme also released sufficient amount of phenolic and hydrophobic compounds. The UV absorption spectrum of the compounds released by enzymatic treatment at 280 nm indicates the presence of lignin in the released coloring matter.
Woman being the root cause of progeny, utmost care should be given to protect her from any ailments that affect her motherhood. PCOD is one of the conditions affecting this unique capacity of woman. The disorder is probably the most common hormonal abnormality in women of reproductive age and certainly a leading cause of infertility. In Ayurvedic classics there is no direct mentioning of this disease rather, symptoms are found under various diseased conditions at various references i.e. menstrual irregularities are described under the broad heading of Ashtoartavadushti, while the description of infertility due to Anovulation is scattered. Pushpaghni, Jataharini mentioned in Kashyapa Samhita, Revati Kalpadhyaya bears similitude to the symptom of hyperandrogenism. But features of metabolic dysfunction and polycystic ovarian morphology are not evident from any description.
Main essence of polymorphism feature of Object Oriented Programming provided by the developer Bjarne Stroustrup in C++ is Run Time Polymorphism using virtual member functions in base classes. Base class pointer keeps the address of derived class object but during run-time it calls the base class function, even functions in base and derived classes have same signatures. To call the same function of derived class by base class pointer, we make the base class function as virtual to implement "Run-Time polymorphism". Several papers by different authors including developer of C++ have come to solve the related complexities like "Object Slicing" and others. In this paper our motivation is to analyse and explore alternative adaptation by type identifier with polymorphic behaviour of type cast combining the overloading of insertion and extraction operators. Analysing the approaches we find a better alternative style of Run-Time Polymorphism that uses less memory than the existing virtual method techniques by comparable performance.
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