The genus Diospyros comprises numerous plant species, several of which are economically and ethnomedicinally important. Some of them yield valuable timber, and some give commercialized edible fruit. Diospyros plants have long been known for their therapeutic property and employed in folk medicine in different parts of the world. Phytochemical study of these plants might give information beneficial for their utilization. Diospyros gracilis is one of Diospyros species with no previous report on phytochemical investigation. In this study, six pentacyclic triterpenoids, including friedelin, epifriedelanol, lupeol, betulin, betulinic acid, and betulinaldehyde, were isolated from the stem of the plant. Identification of the isolated compounds was mainly based on analysis of 1H- and 13C-NMR spectral data, confirmed by comparison with the literature values.