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It was noticed that leucocytes 4 in the circumpallial artery of the oyster at low temperatures tend to form aggregates which gradually become dispersed with elevation of ambient temperatures. On the basis of this observation, a hypothesis was formulated to interpret the fluctuation in the numbers of circulating leucocytes with changes in the ambient temperature in normal oysters. Assuming the number of circulating leucocytes is more or less constant in a normal oyster, to maintain the leucocytes in suspension, a certain amount of agitation must be present in the circulatory system or leucocytes will eventually settle out. Thus, the number of leucocytes in suspension is related to the intensity of agitation. The following series of experiments was designed to test the validity of the above hypothesis.In studies of oyster experimental pathology, sampling of heart blood after intracardial injections of participate or soluble materials is one of the established procedures. To understand the effect of this experimental manipulation on the heart rate and number of 1eucocvte> in the blood is. therefore, of prime importance. M ATKRTAT.S AND METHODS1. O\stcrs, m/iiarta, and sea 7U//

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