Plants were regenerated from tissue cultures of embryos dissected from seeds that were harvested from a selfpollinated clonal selection of Kentucky bluegrass (Poa pratensis L.) 'Baron', an apomictic cultivar. Plants were regenerated from 35 embryo-derived callus cultures of the 3280 embryos that were plated. Flow-cytometric (FCM) and RAPD-marker analyses were performed to determine if regenerants were or were not apomictic in origin. Fifteen regenerants with a 3c DNA content were classified as arising from 2n + n aberrant embryos, which was a higher frequency than expected, based on a chi-square analysis. Of the remaining 20 regenerants with a 2c DNA content, a chi-square test showed that all could have arisen from n + n sexually-derived embryos, based on the observed segregation of n + n regenerants, which fit the expected 3:1 ratio of dominant:recessive RAPD-marker phenotypes. The apparent lack of regenerants of apomictic origin, and implications for genetic transformation and breeding of Kentucky bluegrass are discussed.Abbreviations: n + 0: haploid seedling, 2n + 0: apomictically-derived seedling, n + n: sexually-derived seedling, 2n + n: aberrant seedling derived from unreduced egg and reduced sperm, FCM: Flow cytometry, RAPD: Randomly amplified polymorphic DNA, 1c: haploid, 2c: diploid, 3c: triploid