A perfusion culture system was developed to investigate the oxygenation of high-density hybridoma cell cultures. The culture system was composed of a stirred-tank bioreactor and an external microfiltration hollow fiber cartridge for medium perfusion. Cell growth and antibody production were examined with large bubble ( approximately 5 mm in diameter), micron-sized bubble ( approximately 80 mum in diameter), and silicone tubing oxygenation techniques. Comparable cell growth and monoclonal antibody (MAb) production were found for both the micron-sized and large oxygenation methods, provided that large bubbles were enriched with pure oxygen. Relatively low cell growth and MAb production were attained with the bubble-free silicone tubing oxygenation. It is concluded that direct bubble oxygenation can be applied successfully in high-density animal cell cultures, provided that the culture medium is supplemented with Pluronic F-68. The accumulation of ammonia in the culture medium rather than oxygen limitation was found to be one of the possible problems that eventually inhibited cell growth. This and the fouling of the filtration cartridge during long-term cultivation were found to be more problematic than simple bubble oxygenation of high-density cell culture. The micron-sized bubble oxygenation method is highly recommended for high-density animal cell cultures, provided that Pluronic F-68 is supplemented into the culture medium.

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Measurements have been made in the wake of a model wind turbine in both a weakly unstable and a baseline neutral atmospheric boundary layer, in the EnFlo stratified-flow wind tunnel, between 0.5 and 10 rotor diameters from the turbine, as part of an investigation of wakes in offshore winds. In the unstable case the velocity deficit decreases more rapidly than in the neutral case, largely because the boundary-layer turbulence levels are higher with consequent increased mixing. The height and width increase more rapidly in the unstable case, though still in a linear manner. The vertical heat flux decreases rapidly through the turbine, recovering to the undisturbed level first in the lower part of the wake, and later in the upper part, through the growth of an internal layer. At 10 rotor diameters from the turbine, the wake has strong features associated with the surrounding atmospheric boundary layer. A distinction is drawn between direct effects of stratification, as necessarily arising from buoyant production, and indirect effects, which arise only because the mean shear and turbulence levels are altered. Some aspects of the wake follow a similarity-like behaviour. Sufficiently far downstream, the decay of the velocity deficit follows a power law in the unstable case as well as the neutral case, but does so after a shorter distance from the turbine. Tentatively, this distance is also shorter for a higher loading on the turbine, while the power law itself is unaffected by turbine loading.

A wind-tunnel simulation of an atmospheric boundary layer, artificially thickened as is often used in neutral flow wind-loading studies, has been investigated for weakly unstable stratification, including the effect of an overlying inversion. Rather than using a uniform inlet temperature profile, the inlet profile was adjusted iteratively by using measured downstream profiles. It was found that three cycles are sufficient for there to be no significant further change in profiles of temperature and other quantities. Development to nearly horizontally-homogeneous flow took a longer distance than in the neutral case because the simulated layer was deeper and therefore the length scales larger. Comparisons show first-order and second-order moments quantities are substantially larger than given by 'standard forms' in the mixed layer but are close in the surface layer. Modified functions, obtained by matching one to the other, are suggested that amount to an interpolation in the mixed layer between the strongly unstable and the weakly unstable cases.