The aim of present study was to evaluate the possibility of teratogenicity in rats when exposed to zinc chloride (ZnCl2) pre and post pregnancy. To achieve this goal, a total of 40 mature Albino Wistar female rats were divided equally into four groups as follows: T1, dosed 0.7 mg/day ZnCl2 for two months before mating and till to the day 5th of pregnancy, the females of this group were mated with males dosed 0.7 mg/day ZnCl2 for two weeks before mating; T2, dosed 0.7 mg/day ZnCl2 for two months before mating and till to the day 16th of pregnancy and then were mated with control males (not exposed to any level of ZnCl2); T3, dosed 0.7mg/day ZnCl2 for two months before mating and till the end of pregnancy and were mated with control males; Control, dosed with water free from ZnCl2 along the period of experiment and were mated with control males. At the end of each pregnancy phase, results revealed that alpha fetoprotein serum levels were significantly (P<0.05) higher in all treatment groups compared to the control group, and the most prominent increase was observed in the T3 group. All treatment groups showed a significant (P<0.05) decrease in gestation, viability, and lactation indices when compared to the control group, with the T3 group showing the most significant decrease. Additionally, on days 1, 4, 7, 14, and 21 of lactation period, there was a significant (P<0.05) decrease in mean pup body weights in treated groups compared to the control group, with T3 group having the most prominent body weight decrease. The findings of this study revealed that ZnCl2 at a daily dose of 0.7 mg may cause teratogenic defects in rats at various stages of pregnancy, particularly at the third stage. As high-risk groups, pregnant women and children should use Zn supplementation carefully, whether as a food additive or for self-medication. Simultaneously, evaluating effect of low-dose Zn supplementation over a longer duration is required.
The present study was performed on 30 lactating Iraqi buffaloes suffered from ovarian inactivity diagnosed by rectal palpation during 60-90 days postpartum, aged between 3-6 years at Al-Thahab Al-Abiad village / Abu-Graib / Baghdad province during the period from February- June 2020 . The animals divided into three equal groups (10 buffaloes for each one). 1st group treated with melatonin implants (90mg/animals/SC) for 30 days but 2nd group treated with melatonin implants conjugated with CIDR (1.38 gm) in day 20 for 10 days, 3rd group without treatment (control group). The results in this study were recorded animal response 90%, 100% and 50% in 1st ,2nd and 3rd groups respectively with superior significantly (P<0.01) for treated groups compared with control group and 8.26±1.33, 7.15±0.97 and 160.5±14.31 days which represented the duration of response in 1st ,2nd and 3rd groups respectively with significantly differences (P<0.01) related with control, but the number of services per conception recorded insignificantly in all groups, while the pregnancy rate revealed significant differences (P<0.01) in treated group compared with control group. Finally the days open recorded 153.6±6.72, 142.8±7.45 and 217.3±19.56 in 1st, 2nd and 3rd groups respectively with highly significantly (P<0.01) in treated group compared with control group. In conclusion in present study, the role of melatonin in improvement of reproductive performance in anestrus lactating Iraqi buffaloes suffered from ovarian inactivity was safe and effective
This study was conducted to evaluation of semen quality with the DNA integrity in the season of falcon in Iraq in the different breed (Saker falcon Falco peregrines, and Falco tinnunculus) due to selection of breeding falcons used in the reproduction .the objective of study was to characterize semen quality in the deferent species of falcon. A total of 24 semen sample were collected from males by massage method. Semen parameters include mass activity, individual motility, dead and alive, abnormality and DNA integrity. Mass activity varied between breeds mass activity observed significant (P≤0.05) in the middle period of season, individual motility shows significant between initial, middle and later of season (P≤0.05). The evaluation of viability show no significant between breeds. Some teratospermia recorded, micro-head, tailless, and immature sperms in the different breeds with no significant differences. DNA integrity revealed significant among the individual males (P≤0.05). In conclusion the season of reproduction in Iraq play a role of semen viability and quality between different falcon breeds in Iraq.
This study was conducted to know the effect of ewe age on oocyte quality as well as the relationsbetween oocyte viability and normal uterine condition. Eighty three (83) reproductive systems ofnon-pregnant ewes were collected from Al-shulla abattoir. The Total oocytes were aspirated from right ovaries reached 61.45% and 38.55% from left ovaries. Immediately after aspiration, the oocytes were examined by light microscopic and conceded as mature if surrounded completely with cumulus oopherus. While the stained oocytes by trypan blue were conceded as dead oocytes and excluded. According to ewes age the oocytes were classified into (3) groups, the first group ranged between 1-2 years, second group 3-6 years and the third group over 6 years. The total oocyte collection fromthese groups was 20, 23, 40 oocyte. The results indicated that 14 oocytes (70%), 17(73.91%) and 10(25%) from groups 1, 2, and 3 with cumulus cells, respectively. While the total live oocyte reached to 60. Normal endometrium was observed in 90%, 95% and 80%for 1,2and 3 groups respectively. It was concluded from this study that aged ewes showed low quality oocyte with infertile endometrium.
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