Milk is an ideal food essential for newborns due to its composition and availability. In addition to its macronutrient, i.e. protide, glucide and lipids, milk also contains micronutrients, i.e. vitamins (A, D and B groups) and elements that are absolutely essential during the first months of a baby's life since it is the only source of nutrients 1. Milk contains several elements, which are essential or toxic such as cadmium, lead and nickel, due to these elements presence in the biosphere in general 2. Quantitation of essential and toxic trace elements in milk is an important analytical task, both for human health safety reasons and environmental bio-monitoring purposes. This requires elaboration and application of analytical methods of high sensitivity, selectivity and robustness. But the determination of trace inorganic constituents in milk is not a trivial task because of the complexity of the emulsion. Cow milk contains around 3.4 % of proteins, 2.8 % of casein, 3.7 % of fat and 4.6 % of lactose; additionally, the elements are present as different compounds that can affect both the sample preparation and the measurement strategies 3. Direct procedures for milk analysis were proposed using different spectro-analytical techniques. Each one presents some difficulties where Quinaia and Nobrega 4 , demonstrated that the presence of fat compounds affects the performance of the auto-sampler in graphite furnace

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HPLC method was developed and applied to the determination of some water-soluble vitamins (B1, B2, B3, B6, B9, B12 and C) in Syrian pharmaceutical preparations. Reserved phase, ion-pair high performance liquid chromatography with UV-DAD detection was employed. The developed chromatographic method employed an ODS column with dimension (150 × 4.6 mm; 5 µm). The mobile phase was 5.3 mM of hexane-1-sulfonic acid sodium with 0.1 % triethylamine as solvent (A) at pH 3.3 and 5.3 mM of hexane-1-sulfonic acid sodium:acetonitrile (50:50) with 0.1 % triethylamine as solvent (B) at pH 3.5 and paracetamol as internal standard. The method showed acceptable values for precision, recovery and sensitivity.

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A simple and rapid spectrophotomitric method has been developed to determine orphenadrine citrate and paracetamol in tablet formulations. The first step was based on the reaction of orphenadrine citrate with 1-amino naphthalene and sodium nitrite with heating for 6 min at 50 ºC to give an orange colour having a maximum absorbance at 462 nm. The optimization of the reaction conditions is investigated. In the second step, paracetamol was analyzed after solving it in distilled water by taking the first order derivative spectroscopy and that to eliminate spectral interference with orphenadrine citrate. Beers law is obeyed in the concentration ranges 4.61-80.76 µg/mL and 0.75-30 µg/mL for orphenadrine citrate and paracetamol, respectively. The molar absorptivity, limit of detection, limit of quantification, Sandell sensitivity and linearity are also calculated. The average per cent recovery was found to be 99.90 ± 0.14 % for paracetamol and 99.80 ± 0.05 % for orphenadrine citrate.