Background: Calixarenes are widely used macromolecules in supramolecular chemistry synthesized by simple phenol formaldehyde condensation reaction in the presence of base. This article describes the synthesis, antibacterial, and antifungal properties of calix[4]arene appended with pyrrolidine at the upper rim (CAP3). Methods: Antimicrobial activity of CAP3 was determined by Kirby-Bauer well agar diffusion method in Mueller-Hinton agar (MHA) medium of growth against Escherichia coli (ATCC 8739), Staphylococcus aureus (ATCC 10231) and Streptococcus viridans (ATCC 12392), Candida albicans (ATCC 32333), Aspergillus niger (ATCC 16404), and Aspergillus flavus (ATCC 90906). Structure-activity relationship was also used to evaluate the active site of CAP3.

The present study demonstrates the synthesis and antimicrobial activity of the ptetranitrocalix[4]arene (3). The microbial activity was determined against a variety of microorganisms, i.e., Gram-positive and Gram-negative bacterial strains such as Staphylococcus aureus ATCC 10231, Streptococcus viridans ATCC 12392, Escherichia coli ATCC 8739, as well as some fungal species including Aspergillus niger ATCC 16404, Aspergillus flavus ATCC 90906, and Candida albicans ATCC 32333. Kirby-Bauer well agar diffusion method was employed for the determination of antimicrobial activity. All the microorganisms were applied to a selective agar medium (Mueller Hinton Agar) for growth. It was observed that compound 3 is considerably effective against selected microorganisms. The MIC values were also evaluated. Thus, from the results it could be deduced that compound 3 may be a valuable addition to the therapeutic index.

No abstract