Introduction and Aim: Kruppel Like Factor 14 (KLF14) gene plays an important role in metabolic illnesses and is also involved in the regulation of many other biological processes. This study's objective was to determine whether or not the KLF14 single-nucleotide-polymorphism (SNP) known as rs972283 was linked to an increased risk of peptic ulcer disease in the population that was being investigated.   Materials and Methods: Participants in this study included 71 people who had been diagnosed with peptic ulcers and 50 people who were considered to be healthy controls. In order to genotype the KLF14 SNP rs972283, an amplification refractory mutation system-polymerase chain reaction (ARMS-PCR) was carried out, and the PCR results were then sequenced.   Results: Genotypes (GG, AG, and AA) were significantly different in people who had peptic ulcers compared to those who were in the control group (chi-square=7.703, 5.317 and 4.294) respectively. AG and AA genotypes for KLF14 gene were correlated with a high risk of peptic ulcer (P 0.05) (Odds Ratio (O.R.) =6.343 and 2.441) respectively. Patients with peptic ulcer had a significantly greater incidence of the allele A gene (30.3%), whereas healthy people had a much higher incidence of the G allele (86%). Patients who carried the AG genotype and had a chronic H. pylori infection were found to have a highly significant correlation with one another (P 0.01, O.R. =1.218). Similarly, there was a higher frequency of the G allele (84.6%), in people who had peptic ulcers, but there was a higher frequency of the A allele (39%), in cases of chronic infection.   Conclusion: According to the findings of this research, a variant in the KLF14 gene called rs972283 is linked to an increased risk of peptic ulcer illness.