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Background: Psoriasis is a genetically regulated autoimmune skin disease associated with the impaired suppressive function of Treg cells. Foxp3 is the accepted marker of Treg cell, but CD127 (IL-7Rα), a surface marker of activated T cell, can distinguish the functional status of CD4+CD25+FoxP3+Treg cells. The study aimed to demonstrate the functional status of Treg cells in peripheral blood of psoriasis patients, to analyze their association with disease severity and duration, serum IL-17 level, and IL-23R gene polymorphism to observe the relationship with susceptibility to psoriasis. Methods: Thirty-five psoriasis patients and 35 healthy controls were enrolled. Demographical details were recorded, severity was assessed by Psoriasis Area and Severity Index (PASI) scoring. Treg cells were quantified by flowcytometric immunophenotyping, serum IL-17 level by ELISA, and IL-23R gene polymorphism by Real-Time PCR. Results: Increased circulatory CD4+CD25+FoxP3+Treg and CD4+CD25+FoxP3+CD127+/pTreg cells observed in psoriatics than in controls (P<0.05) but CD4+CD25+FoxP3+CD127-/tTreg cells significantly decreased (P<0.001). IL-17 levels were elevated in psoriatics (P=0.002). In severe patients, FoxP3 expression decreases in tTreg cells and are CD127+. Out of 35 patients, 23 (65.71%) had an AA genotype of IL23Rrs10889677 gene polymorphism, associated with increased Treg, tTreg, pTreg cells, and IL-17 levels revealed susceptibility to psoriasis. Conclusion: This study shows the loss of tTreg function and pTreg cell activation in psoriasis, supporting the autoimmune disease model. CD127 expression can better distinguish Treg cells and augmenting the CD127-/low population of Treg cells to restore suppressive activity by anti-CD127 mAb therapy can be a potential treatment option for psoriasis patients.

Introduction: Psoriatic arthritis (PsA) is established as a multifactorial disease resulting from a complex interplay between genetic, environmental and immunological factors. It is a seronegative arthritis but rheumatoid factor may be present in up to 15% of PsA patients Antibodies recognizing a cyclic citrullinated peptide are highly speciûc for rheumatoid arthritis (RA) but their role in PsA remains unclear. An increased prevalence of anti-CCP antibody in PsA is also reported. Study shows that HLA-DRB1 shared epitope is signiûcantly associated with the presence of anti-CCP antibody in PsA patients but this type of association is not found with other human leukocyte antigens. Objectives: The aim of this study was to investigate the frequency of anti-CCP and RF in PsA patients and their associations with HLA-B locus antigens. Methods: In this cross sectional study, we selected 50 unrelated consecutive patients with PsA according to CASPAR criteria for PsA. 6 ml of blood was collected from each patient for HLAB locus typing, RA test and test for anti-CCP. Patient’s serum samples were tested for RF by Nephelometric system and tests for anti-CCP were done by ELISA. HLA-B locus typing was done by PCR with sequence specific primer. Results: Among 50 PsA patients, 27 (54%) are female and 23 (46%) are male. RA test is positive in 10 (20%) patients and anti-CCP is positive in 7 (14%) patients. Significant association was found between HLA-B*37 and RF (p value= < 0.001). Conclusion: RF is present in 10 (20%) and anti-CCP is present in 7 (14%) PsA patients. HLAB* 37 was significantly found in RF positive patients. J Shaheed Suhrawardy Med Coll 2020; 12(2): 109-114