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<p class="Default">In Morocco, Raphanus sativus is a widespread traditional medicinal plant used to treat various kidney diseases such as nephropathy.</p><p class="Mabstract"><strong>Objective</strong>:<strong> </strong>The present study aims to evaluate the protective effect of the <em>R. sativus </em>aqueous extract against the gentamicin-induced acute nephrotoxicity in rats.</p><p class="Mabstract"><strong>Methods</strong>: Rats were randomly separated into four groups (n=6; ♂/ ♀=1). The control group was treated only with distilled water (10 mL/kg; <em>p.o</em>). The gentamicin group was treated with distilled water (10 mL/kg; <em>p.o</em>) and injected intraperitoneally by the gentamicin (80 mg/kg; <em>i.p</em>). <em>R. sativus </em>groups were treated with the aqueous extracts of this plant at a dose of (200 or 400 mg/kg; <em>p.o</em>) and injected by the gentamicin (80 mg/kg; <em>i.p</em>). The plasma Creatinine, Urea, Uric Acid, Albumin, Total Protein, Alanine transaminase, Aspartate transaminase, and Calcium levels were measured. The urinary creatinine, urinary Calcium, urinary volume, water intake, creatinine clearance, body weight gain, relative right kidney weight, and kidney malondialdehydes were determined.</p><p class="Mabstract"><strong>Results</strong>: This study showed that the daily pretreatment with <em>R. sativus</em> aqueous extract at two doses of 200 and 400 mg/kg, p.o prevented the rats from the gentamicin-induced nephrotoxicity. Moreover, the <em>R. sativus </em>aqueous extract showed a high amount of polyphenols and flavonoids and a significant antioxidant activity.</p><p class="Mabstract"><strong>Conclusion</strong>: <em>R. sativus </em>aqueous extract, as a novel natural product, may have preventive properties against gentamicin-induced nephrotoxicity in rats. The present study describes new areas of investigation to introduce better therapeutic agents for renal disorders and dysfunction.</p>

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The present study investigated the antioxidant activity, metal chelating ability and genoprotective effect of the hydroethanolic extracts of Crocus sativus stigmas (STG), tepals (TPL) and leaves (LV). We evaluated the antioxidant and metal (Fe2+ and Cu2+) chelating activities of the stigmas, tepals and leaves of C. sativus. Similarly, we examined the genotoxic and DNA protective effect of these parts on rat leukocytes by comet assay. The results showed that TPL contains the best polyphenol content (64.66 µg GA eq/mg extract). The highest radical scavenging activity is shown by the TPL (DPPH radical scavenging activity: IC50 = 80.73 µg/mL). The same extracts gave a better ferric reducing power at a dose of 50 µg/mL, and better protective activity against β-carotene degradation (39.31% of oxidized β-carotene at a 100 µg/mL dose). In addition, they showed a good chelating ability of Fe2+ (48.7% at a 500 µg/mL dose) and Cu2+ (85.02% at a dose of 500 µg/mL). Thus, the antioxidant activity and metal chelating ability in the C. sativus plant is important, and it varies according to the part and dose used. In addition, pretreatment with STG, TPL and LV significantly (p < 0.001) protected rat leukocytes against the elevation of percent DNA in the tail, tail length and tail moment in streptozotocin- and alloxan-induced DNA damage. These results suggest that C. sativus by-products contain natural antioxidant, metal chelating and DNA protective compounds, which are capable of reducing the risk of cancer and other diseases associated with daily exposure to genotoxic xenobiotics.