Background: Uncertainty about any kind of medication will be a potential threat. Hesitancy regarding vaccination is a danger to overall global public health. Since it’s the first-time global efforts were made to develop a vaccine against the pandemic of COVID-19. So, this is unknown its acceptance in the community. Getting information regarding the key determinants that influence and potential myths about the vaccine might help full to develop strategies that can help improve the vaccination programs globally. This study aimed to assess the prevalence of the acceptance of the COVID-19 vaccine, its determinants, and myths about the vaccine among people in Pakistan. Methods: A descriptive web-based based study was done, using snowball sampling, bilingual, and a self-administered close-ended questionnaire was sent to participants through google forms on social media platforms and email. Results: A total of 463 participants from all over Pakistan showed the minimum level of secondary education. The occupational status of the participants showed that (45.1%) were currently employed. Vaccination and their perception can be influenced by educational/socioeconomic statuses, participants were also asked about factors they considered to be the most influential in terms of encouraging and discouraging. Fear of getting an infection was the most encouraging factor (39.1%) for people to get vaccinated, whereas adverse effects via social and digital media were the most demotivating factor (36.5%).  Participants were asked the reasons for their reluctance; most were afraid of an allergic reaction to the vaccine. The various myths and misconceptions associated with the Covid vaccine showed by logistic regression analysis for factors that were potentially associated with the intake of covid vaccination. Conclusion: Addressing educational/sociodemographic factors relating to the COVID-19 vaccination might help encourage the uptake of the vaccination program globally which also tackles future pandemics.