Population studies on six short tandem repeat loci, HUMF13A01, HUMFXIIIB, HUMLIPOL, HUMTH01, HUMTPOX and HUMVWFA31 were carried out in a sample of unrelated Japanese individuals (n = 337-545) living in Gifu Prefecture (central region of Japan). Five alleles could be identified for HUMFXIIIB, six for HUMF13A01, HUMLIPOL, HUMTH01 and HUMTPOX, and eight for HUMVWFA31. For all/six loci no deviations from the Hardy-Weinberg equilibrium hypothesis were detected. The mean exclusion chance ranged from 0.22 to 0.60, the power of discrimination from 0.63 to 0.93, and the expected heterozygosity from 0.43 to 0.80. Allele frequency distributions for the loci in the Japanese sample were not similar to those in samples from other racial or ethnic groups except for the Chinese (for HUMTPOX). The results demonstrate that HUMTH01, HUMTPOX and HUMVWFA31 are more useful for forensic investigations in the Japanese population that the other three loci.

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has been known that hemolysate is composed of not only hemoglobin but also non-hemoglobin proteins (NHP). NHP isolated effectively from hemolysate of adult and newborn by CM-Sephadex chromatography was investigated by polyacrylamide disc or slab electrophoresis. It was demonstrated on slab electrophoresis that adult NHP is composed of 4 zones (approximately 14 bands) with different mobilities and that adult NHP shows a considerably different pattern from cord NHP. The difference was observed also on the electrophoresis stained for esterase or lactate dehydrognease. Rabbit antiserum to adult NHP was adsorbed with cord NHP and specific antiserum for adult NHP was prepared.However, antiserum reacting with only cord NHP was not obtained from anti-cord NHP serum. These results suggested that adult specific components with passable antigenicity were present in adult NHP and that cord NHP had similar antigenicity as adult NHP.non-hemoglobin protein; adult specificity Recent progress in separating techniques such as electrophoresis and column chromatography demonstrates that hemolysate is composed of many constitutents. Stern et al. (1945) pointed out by Tiselius electrophoresis that hemolysate is composed of not only hemoglobin but also many non-hemoglobin proteins (NHP). Haut et al. (1962) reported that about 6% of hemolysate was estimated to be NHP. Moreover, Haut et al. (1964) revealed subsequently that some NHP zones on the starch gel electrophoresis had esterase, lactate dehydrogenase or glucose-6-phosphate dehydrogenase activity. Recently the significance of those constituents has been biochemically and genetically elucidated. In this paper, polyacrylamide disc or slab electrophoresis, of which resolution was much higher than other electrophoresis methods, was adopted to analyze NHP of adult and newborn, and adult specific components in NHP were immunologically investigated. MATERIALS AND METHODSPreparation of hemolysate. Blood samples from healthy adults and cord were washed five times with five-fold volumes of saline. Hemolysate was prepared by lysing the cells

The effect of hair dyes, i.e., temporary, semi-permanent, or permanent hair dyes, or hair bleach on the isoelectric focusing (IEF) hair protein patterns was studied. A permanent hair dye (metallic, alkaline oxidative, or acidic oxidative) and hair bleach induced changes in the IEF hair protein patterns and in the intensity of hair protein bands. The changes in the IEF patterns, caused by the alkaline oxidative dye or the bleach, are considered to result from the combined effect of an alkaline agent and an oxidative agent in the alkaline oxidative dye and in the hair bleach.