BackgroundThe Eurasian grapevine (Vitis vinifera L.) is the most widely cultivated and economically important horticultural crop in the world. As a one of the origin area, Anatolia played an important role in the diversification and spread of the cultivated form V. vinifera ssp. vinifera cultivars and also the wild form V. vinifera ssp. sylvestris ecotypes. Although several biodiversity studies have been conducted with local cultivars in different regions of Anatolia, no information has been reported so far on the biochemical (organic acids, sugars, phenolic acids, vitamin C) and antioxidant diversity of local historical table V. vinifera cultivars grown in Igdir province. In this work, we studied these traits in nine local table grape cultivars viz. ‘Beyaz Kismis’ (synonym name of Sultanina or Thompson seedless), ‘Askeri’, ‘El Hakki’, ‘Kirmizi Kismis’, ‘Inek Emcegi’, ‘Hacabas’, ‘Kerim Gandi’, ‘Yazen Dayi’, and ‘Miskali’ spread in the Igdir province of Eastern part of Turkey.ResultsVariability of all studied parameters is strongly influenced by cultivars (P < 0.01). Among the cultivars investigated, ‘Miskali’ showed the highest citric acid content (0.959 g/l) while ‘Kirmizi Kismis’ produced predominant contents in tartaric acid (12.71 g/l). The highest glucose (16.47 g/100 g) and fructose (15.55 g/100 g) contents were provided with ‘Beyaz Kismis’. ‘Kirmizi Kismis’ cultivar had also the highest quercetin (0.55 mg/l), o-coumaric acid (1.90 mg/l), and caffeic acid (2.73 mg/l) content. The highest ferulic acid (0.94 mg/l), and syringic acid (2.00 mg/l) contents were observed with ‘Beyaz Kismis’ cultivar. The highest antioxidant capacity was obtained as 9.09 μmol TE g-1 from ‘Inek Emcegi’ in TEAC (Trolox equivalent Antioxidant Capacity) assay. ‘Hacabas’ cultivar had the highest vitamin C content of 35.74 mg/100 g.ConclusionsPresent results illustrated that the historical table grape cultivars grown in Igdir province of Eastern part of Turkey contained diverse and valuable sugars, organic acids, phenolic acids, Vitamin C values and demonstrated important antioxidant capacity for human health benefits. Further preservation and use of this gene pool will be helpful to avoid genetic erosion and to promote continued agriculture in the region.
Mulberries, Morus spp., grow in different growing regions of Turkey with a high diversity providing an opportunity to select superior accessions. The goal of the study was to determine the differences in phytochemical components and horticultural characteristics between white and black mulberry accessions in the Igdir province. Fruits of black and white mulberries collected from Igdir province were assayed for various horticultural characteristics including berry width, berry length, berry weight, and berry peduncle length. Samples were also assayed for vitamin C, organic acids (citric, tartaric, malic, succinic, and fumaric), sugars (glucose and fructose), phenolic acids (catechin, rutin, quercetin, chlorogenic, ferulic, o-coumaric, p-coumaric, caffeic, syringic, vanillic, and gallic acids), and antioxidant capacity [Trolox equivalent antioxidant capacity (TEAC) assay]. Differences (P < 0.05) were noted between accessions in berry length and berry weight. Malic acid was found to be the predominant organic acid regardless of species. Rutin (for black mulberry) and chlorogenic acid (for white mulberry) were the predominant phenolic acids. Black mulberry had higher glucose and fructose content than white mulberry. Analysis showed that black mulberry had higher content of tartaric acid, malic acid, TEAC, fructose, glucose, catechin, rutin, quercetin, o-coumaric acid, and caffeic acid compared with white mulberry (P < 0.01); however, white mulberry had higher content of succinic acid, vitamin C, chlorogenic acid, ferulic acid, p-coumaric acid, syringic acid, and gallic acid (P < 0.01). Our results provide a reference for further studies of mulberry fruits in detection of organic acids, sugars, antioxidant capacity, and phenolic compounds. Also, it is clear that we need standardized testing, extraction, and analysis protocols to compare genotypes grown in different countries.Key words: antioxidant capacity, mulberry, organic acids, phenolic compounds.Résumé : La mûre, Morus spp., pousse dans plusieurs régions de la Turquie et présente une grande diversité, ce qui pourrait conduire à la sélection de variétés supérieures. L'étude devait établir les différences des obtentions de mûre noire et blanche glanées dans la province d'Igdir au niveau des composants phytochimiques et des propriétés horticoles. Les auteurs ont précisé les paramètres horticoles des mûres noires et blanches récoltéés dans cette province, notamment la largeur et la longueur du fruit, son poids et la longueur du pédoncule. Ils ont aussi dosé la concentration de vitamine C, des acides organiques (citrique, tartrique, malique, succinique et fumarique), des sucres (glucose et fructose) et des acides phénoliques (catéchine, rutine, quercétine, acide chlorogénique, férulique, o-coumarique, p-coumarique, caféique, syringique, vanillique et gallique) ainsi que le potentiel antioxydant [test « Trolox equivalent antioxidant capacity » (TEAC)] des échantillons. Des écarts (P < 0,05) ont été relevés entre les obtentions au niveau de la longueur et du...
Continuous seed propagation in Turkey has given rise to a great number of seedling walnut trees which represents valuable walnut genetic resources. The number of native walnut trees is estimated to be over 5 million in Turkey and they possess large phenotypic variability in yield, nut and kernel characteristics, late bud breaking, late flowering, winter hardiness and tolerance to diseases. Progress in walnut breeding requires the exploitation of genetic variation among cultivars and landraces. In this study, we used 32 local diverse walnut genotypes obtained from seeds and 2 standard cultivars (‘Sebin’ and ‘Bilecik’). This study implemented 21 previously used simple sequence repeats (SSR) markers to determine genetic diversity. The analysis revealed 135 alleles with an average of 6.43 alleles per locus. Genetic similarity ranged from 0.23 (for samples KW22 and KW29) to 0.87 (for samples KW27 and KW28). The highest number of alleles per locus was obtained from WGA276 locus (11 alleles), followed by WGA054 (9 alleles), WGA202 and WGA321 (8 alleles) while the lowest number was detected in WGA027. According to the morphological and molecular data, the genotypes differed from each other and the cvs. Sebin and Bilecik. The majority of the genotypes had higher fruit weight and some of the genotypes had higher kernel ratio than cvs. Sebin and Bilecik implying the importance of registering genotypes as cultivars. This research provides information on the genetic relationship of walnut genotypes and cultivars and emphasises the importance of protection and utilisation of seed-propagated walnut genetic resources.
Sugars (glucose and fructose), organic acids (citric acid, tartaric acid, oxalic acid, malic acid, succinic acid and fumaric acid), vitamin C, phenolic compounds (catechin, rutin, quercetin, chlorogenic acid, ferulic acid, o-coumaric acid, p-coumaric acid, caffeic acid, syringic acid, vanillic acid and gallic acid) and antioxidant capacity (Trolox equivalent antioxidant capacity, TEAC assay) were determined in fruits of wild blackberry, black and white mulberries from southern Bulgaria. Malic acid was the predominant organic acid for the black and white mulberries. Citric and malic acids were represented with the highest content in blackberries. The highest fructose concentration was observed in blackberries (16.187 g¢100 g ¡1). Black mulberries showed the highest antioxidant activity (12.230 mmol TE g ¡1), followed by white mulberries (8.6133 mmol TE g ¡1) and blackberries (4.490 mmol TE g ¡1). Chlorogenic acid and rutin were the main phenolics in the analysed fruits. The results illustrated significant phytochemical profiles of the studied berries, which could contribute to the medicinal industry and provide valuable genetic resources for breeding programmes.
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