In this study, the cell-wall microstructure and anti-swelling effectiveness (ASE) of heat-treated larch wood were evaluated and the correlation between them was analyzed. For this purpose, some larch lumbers were heat-treated for 12, 18, and 24 hours at temperatures of 190°C and 220°C. By observing the scanning electron microscopy cross-sectional image of the heat-treated larch, it was confirmed that the shape of heat-treated wood cell changed, the cut-section of the wood cell wall was rough, and the intercellular space has become wide as the intercellular bonds had broken because of heat-treatment. In addition, the evaluation of the swelling for each treatment condition revealed that, as the heat-treatment temperature and duration increased, the amount of absorbed water and swelling decreased and the ASE increased. The decrease in the amount of absorbed water is thought to be affected by the chemical change in the cell wall by heat-treatment. On the contrary, the decrease in the swelling and the increase in the ASE are thought to be due to a combination of chemical changes and physical changes such as structural changes in the cell wall.
In this study, we investigated the applicability of a termiticide based on metofluthrin for termite control on traditional wooden buildings. To evaluate their mortality, termites were exposed to the agent, diluted to various concentrations, for seven days; it was found that it had a sufficient insecticidal effect although there was a difference depending on the degree of agent dilution. Next, the effect on Dancheong, used in traditional wooden structures, was measured based on the color change according to the number of agent treatments. The results showed that only the color difference value of two pigments (i.e., Bun and Juhong) was measured as 3.0 or higher. However, there this varied according to the dilution ratio. Thus, it was found that most pigments had little effect on the color of Dancheong. Finally, the termiticidal efficacy of the agent when applied to wood was evaluated. We found that the specimens coated with 10-fold and 20-fold dilutions exhibited 2.83 and 6.28% mass loss, respectively. The 10-fold dilution satisfied the performance of termiticide as a mass loss of less than 3%. In conclusion, the metofluthrin-based agent used in this study has little effect on various Dancheong pigments and has a termiticidal effect against termites; it is therefore suggested that it may be used in preserving traditional wooden cultural properties in Korea.
The mechanical properties and termite and decay resistance performance of wood-based panel used in wooden houses were evaluated. The physical and mechanical properties, along with the resistance levels, of commonly used wood-based panels, including oriented strand board, structural particleboard, and particleboard for use in interior, were compared. The structural particleboard complied with the physical, mechanical, and formaldehyde emission standards of the International Organization for Standardization and Japanese Industrial Standard, surpassing the requirements for oriented strand board. The structural particleboard exhibited excellent water resistance and a consistent performance. Decay tests classified the particleboard and structural particleboard as “Resistant,” with mass losses of 7.82 to 12.72% (white rot) and 14.69 to 16.55% (brown rot). Pine (Pinus densiflora) and oriented strand board exhibited no decay resistance, with mass losses exceeding 45%. The particleboard and structural particleboard demonstrated superior termite resistance, resulting in 100% termite mortality in three days without chemical treatment. The structural particleboard exhibited excellent water, decay, and termite resistance, which can be an advantage in wooden-house construction in terms of maintenance.
Acanthophysium sp. KMF001, a wood rotting fungus, produces a strong crude enzyme complex that efficiently produces simple sugars from wood. The transcriptomic analysis of Acanthophysium sp. KMF001 identified 14 genes for putative glycoside hydrolases. Among them, isotig01043 was expressed heterogeneously in Escherichia coli BL21(DE3), and the expressed protein exhibited an endo-β-1,4-xylanase activity which showed the optimum reaction at pH 5.0 and 30°C. The enzyme kinetic values of K m and V max were 25.92 mg/ml and 0.628 µmole/mg/ml, respectively. The enzymatic characteristics of the expressed xylanase showed a typical fungal xylanase. However, the bioinformatics analysis suggested that the protein encoded by isotig01043 was a novel xylanase based on a low identity when it was compared with the closest protein in the NCBI database and a similar protein domain with GH16_fungal_Lam16A_glucanase, which had not been earlier suggested as a xylanase.
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