The creation of therapeutic monoclonal antibodies has increased recently. mAbs have grown significantly in relevance, and these compounds are now more crucial than ever in the global biotechnological drug industry. For the treatment of various diseases, a lot of biotechnology companies are investing in the manufacture of monoclonal antibodies. The two main processes in the synthesis of these antibodies are bioreactor production and purification. This article goes into great detail on the current purification techniques utilized for these compounds. The fundamental monoclonal antibody recovery and purification procedures, including cell harvesting, Protein A affinity chromatography, and further polishing steps, are all enumerated.