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Rheology of MHD bioconvective nanofluid containing motile microorganisms is inspected numerically in order to analyze heat and mass transfer characteristics. Bioconvection is implemented by combined effects of magnetic field and buoyancy force. Gyrotactic microorganisms enhance the heat and transfer as well as perk up the nanomaterials’ stability. Variable transport properties along with assisting and opposing flow situations are taken into account. The significant influences of thermophoresis and Brownian motion have also been taken by employing Buongiorno’s model of nanofluid. Lie group analysis approach is utilized in order to compute the absolute invariants for the system of differential equations, which are solved numerically using Adams-Bashforth technique. Validity of results is confirmed by performing error analysis. Graphical and numerical illustrations are prepared in order to get the physical insight of the considered analysis. It is observed that for controlling parameters corresponding to variable transport properties c2, c4, c6, and c8, the velocity, temperature, concentration, and bioconvection density distributions accelerates, respectively. While heat and mass transfer rates increases for convection parameter and bioconvection Rayleigh number, respectively.