Background: Since worries about not harming patients have gotten more attention in recent years, patient safety has become a key healthcare priority. Numerous studies have found that adverse outcomes are more likely to occur in general healthcare settings. Aim: the current aim is to assess perceptions of staff nurses about patient safety culture at Minia General Hospital. Research design: descriptive cross-sectional research design. Setting: The study was conducted in Minia General Hospital, at Minia city, Egypt. Subjects: convenience sample included all available staff nurses during data collection in Minia General Hospital (no=280). Tool of data collection: one tool was used Patient Safety Culture. Results: the study showed that the highest percentage of staff nurses have high total scores regarding patient safety culture for the dimensions "feedback and communication about error", "frequency of event reporting" "teamwork within units" and "organizational learning continuous improvement". Also, the study showed that the lowest dimensions from the staff nurses perception were for "supervisor \ manager expectations and actions promoting patient safety"; "overall perceptions of patient safety" "staffing"; and "teamwork across hospital units". Conclusion: the results indicated that the overall perception of staff nurses regarding patient safety culture in the hospital were moderate. Recommendation: nurses should be encouraged to participate and foster the patient safety activities and communication channels in order to maintain safety regulation