Transmission electron microscopy (TEM) was employed for describing skin and scab lesions in goats affected by orf virus and to demonstrate the parapoxvirus from clinical suspensions by negative staining and ORFV confirmation by immunogold electron microscopy. All samples were confirmed as parapoxvirus by semi-nested PCR amplification of partial gene encoding for the B2L envelope protein. Skin lesions were characterized by ballooning degeneration and loss of desmosomes of the spinosum cells, cytolysis and vesicle formation. Nuclear changes included chromatin margination and increase in electron density. Cytoplasmic changes were typical of cell swelling, vacuolation and the presence of uniform, moderately electron dense viroplasm, situated in the perinuclear region. Various intracellular forms including immature virions (IV), mature virions (IMV) and wrapped virions (WV) were observed in the cytoplasm. All these forms of ORFV were observed morphologically akin to vaccinia virus (VACV). Negative staining of clinical samples and viral suspensions showed typical parapoxvirus morphology with a characteristic criss-cross tubular surface

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Aim:Immune response to a lyophilized peste-des-petits-ruminants virus (PPRV) vaccine was evaluated in three different breeds of goats.Materials and Methods:Three breeds of goats consisting six number of animals in three groups, i.e., Group A (local Assam hill goat), Group B (cross-bred), and Group C (Beetal goats) were randomly selected for evaluating the immune response to a lyophilized PPRV vaccine.Results:A higher rise in the overall mean serum antibody titer was observed in Group A (40.50±3.74) than in Group B (37.58±37.58) and Group C (35.90±3.29) during the study period.Conclusion:Initially, a negative PPRV specific serum antibody titer was recorded in all the groups at 0th day of vaccination. Serum antibody titer in the vaccinated goats started rising gradually from the 14th day post vaccination. Later higher rise in the overall mean serum antibody titer in Group A (local Assam hill goat) lead to the conclusion that higher serum antibody titer in local non-descript breed might be due to their better adaptation to the environmental condition.

Highland pastoralism provides economic sustainability to the tribal livelihoods and is endemic to the yak-rearing tracts of India. Transhumant pastoralists of Arunachal Pradesh (in India) have centuries-old deep socio-religious and economic connections with this unique bovine species. As a result of their conservation efforts, Arunachali was recognized as the first and is still the lone breed (to date) of yaks in the country. A survey was conducted on the pastoral production system in the region to enable the phenotypic characterization of yaks and to understand the prevailing husbandry practices. Arunachali yaks are medium-sized bovines that are predominantly black with dense and long hairs hanging down the body and are docile in temperament. They have a convex head with horizontal ears and distinctly curved horns with pointed tips. The average milk yield is 0.98–1.04 kg milk/day with 7.45% fat and 11.5% SNF and the peak milk yield/day is 1.1–1.6 kg. The average ages of clipping of coarse hairs and down fibres are 12–18 months and 12 months, respectively, with average yields of 1.5 kg and 0.5 kg/clipping/animal, respectively. Value addition of yak milk and fibre presents a unique opportunity for the economic rejuvenation of yak pastoralism. However, winter feed scarcity, inbreeding, extreme climate events and the non-availability of essential services are still major challenges for yak production in the country. Our findings acknowledge that pastoral sustainability is critical for the conservation of yaks and yak rearing. This calls for pastoralism-centric governance and research efforts in the highlands to curb the declining population and to put Indian yaks on the road to sustainability.

The Arunachali breed of yak is reared by the Monpa pastoral community of Arunachal Pradesh in India for their livelihood and nutritional security on highland pastures. In addition to fulfilling the basic needs of highlanders, this animal is associated with poor production potential linked to its inherent reproductive problems, including silent oestrus, seasonality, and inbreeding, making yak rearing a less-profitable venture. In the recent past, efforts have been made to improve reproductive efficiency of Arunachali yak through assisted reproductive techniques. However, a few regimens for superovulation have been tried to date with limited success in terms of superovulatory response and embryo recovery. Therefore, the present study was undertaken to evaluate the efficacy of two different doses and regimes of superovulation: Stimufol (400 and 200µg per animal) and Folligon (1500 and 1000IU per animal) in Arunachali yak. Twenty-four yaks were allotted in 4 groups (of 6 animals each) and subjected to respective superovulation treatment. Thereafter, the treatment response was assessed in terms of expression of oestrus, interval between treatment and oestrus onset, duration of oestrous, number of corpora lutea and embryos recovered, and analysed using two-way statistical analysis between treatment groups. The results (Table 1) showed a significant (P<0.01) variation in onset of oestrus after the treatment, whereas other responses did not differ among the groups. Further, the recovered embryos were categorized as excellent, good, fair, and poor according to cell size, shape, texture, and number of vesicles present in the blastomeres; 58.33, 20.83, 8.33, and 12.5% embryos in these categories were recovered, respectively. The overall embryo recovery rate was 30.77%, ranging from 10.53 to 52.38% in different treatment groups. The numbers of embryos recorded were less than the numbers of CL present, showing the possibility of non-availability of all ovulated oocytes for fertilization in the oviduct due to untrapping by the fimbriae. The effectiveness of treatments depended upon the precision of oestrus detection and the time of ovulation. In our study, the variation in onset of oestrus might be associated with poor expression of behavioural oestrus in the treated animals. Based on the present findings, we conclude that all the treatments are effective in superovulation and embryo recovery. However, the use of Folligon at 1500IU seemed to be comparatively more effective in Arunachali yak. Table 1. Mean (n=6) average of treatment responses in yak following different superovulatory treatment Variable Treatment Stimufol Folligon 400 µg/animal 200 µg/animal 1500 IU/animal 1000 IU/animal Oestrus response (%) 100 100 100 100 Interval between treatment and oestrus onset (h) 17.67±0.56a 20.83±0.79b 21.17±0.79bc 23.67±1.43c Duration of oestrous (h) 30.33±1.09 28.50±0.99 31.50±1.50 28.83±1.25 Corpora lutea (n) 2.83±1.22 3.50±1.18 3.50±0.67 3.17±0.87 Embryo recovered (n) 0.83±0.83 1.00±0.81 1.83±0.98 0.33±0.21 Recovery rate (%) 29.41 28.57 52.38 10.53 a–cMeans within a row with different superscripts differ P<0.05.