Molecular modeling calculations were used to validate 3D structures of new complexes of Ru(III), Pd(II) and VO(II) ions chelated with (E)-2-(phenylamino)-N-(pyridine-2-yl)methylene)acetohydrazide ligand. Furthermore, the calculations were used to estimate selected electronic chemical descriptors which are responsible for the biological activity. The first insight of the compound activity as antibacterial was evaluated by molecular docking analysis. The titled models showed stable binding towards lanosterol 14 alpha-demethylase (CYP51) enzyme of E. coli, indicating their inhibition effect toward bacterial growth. Structural study of the ligand and Ru(III), Pd(II) and VO(II) chelates was done using elemental analysis, FT-IR, 1H-NMR techniques. Furthermore, complexes were physically investigated based on magnetic moment, molar conductance, electronic spectroscopic and thermal analysis techniques. The antibacterial study of the synthesized compounds screened against both Gram-positive and Gram-negative bacteria revealed that these compounds display remarkable antibacterial activity and can be used as therapeutic drugs for pathogenic bacterial diseases. All complexes and ligand showed good scavenging activities which indicate a promising result for their applications as antioxidants.

Some microorganisms, i.e., Rhizobium leguminosarum var. faba, Aphanocapsa albida and Laurencia obtusa, were applied in pots to enhance the growth of faba bean (Vicia faba L.) plants. The individual and combined microorganisms applications significantly increased the plant height and number of leaves at the early growth stages. Ninety days after planting, all the treatments significantly enhanced the plant growth parameters. Laurencia obtusa + Aphanocapsa albida increased the whole plant length, dry weight, total phenols content and nitrogen content by 46.3%, 96.5%, 315% and 86% respectively. However while the combination between the three microorganisms increased whole plant fresh weight by 56.0% in comparison with control.

Some biofertilizers agents i.e. Rhizobium leguminosarum var. fabae, Bacillus megaterium var., phosphaticum and Trichoderma harizianum play important roles in enhancing the plant growth and controlling several diseases i. e. root rot and stem canker disease. In this work, under greenhouse conditions, Rhizoctonia solani caused dampingoff and death of all faba bean seedlings, however the three tested microorganisms resulted good biocontol role against the pathogenic fungus and the application of T. harizianum gave the best result in this trend. The treatment of T. harizianum and R. leguminosarum var. fabae to the soil infested with Rhizoctonia solani showed significant increase in leaves number of faba bean plants compared with the untreated plants or which treated plants or which treated with Bacillus megaterium var., phosphaticum after 40 days from planting. Application of T. harizianum and R. leguminosarum var. fabae gave the best plant growth while the presence of the pathogenic fungus showed significant decrease in fresh weight, dry weight and nodules number on roots of faba bean plants. Also, polyphenols and antioxidants contents in the shoots and roots were decreased in the presence of the pathogen compared with the untreated plants. Application of R. leguminosarum resulted significant increase in the roots and shoots total nitrogen and protein.

Understanding the impacts of seasonal variations on fate and distribution of natural and introduced microbes are important for proper public health, risk assessment and water management of Lake Burullus, Egypt (LB) as water receiving body. The effects of mixing processes on microbial loads in Lake and discharged aquacultural wastewater (AQ) and agricultural drainages (AG) were assessed. Microbiological examinations of discharged waters in this study revealed that total bacterial count (TBC), and total coliform count (TCC) were significantly high at P 0.05 in aquacultural wastewater than both LB and AG. Total fecal coliform count (TFC) and total fecal streptococcus (TFS) were determined and showed that AG has significantly high at P 0.05 comparing to both LB and AQ. No seasonal differences in-between all indictors values of LB, while AG wastewater values were mostly higher than both AQ and LB water samples with declining attitude between sampling points. In case of TBC, TCC and TFC, the microbial loads of lake water were highly affected by receiving water but in case of TSF, the water shows no affects. Contrary, for discharged waters, both show reduction in count that is meaning of dilution processes. As excessive discharge of raw wastewater may present a risk related to high increased rate of TBC and TCC in lake water annually by 27.8 and 33.0% at 500 m points, respectively.

The enormous industrial usage of nickel during its manufacture and recycling has led to widespread environmental pollution. This study was designed to examine the ability of Gelidium amansii biomass to biosorb Ni2+ ions from an aqueous solution. Six independent variables, including contact time (1.0 and 3.0 h), pH (4 and 7), Ni2+ concentration (25 and 200 mg·L−1), temperature (25°C and 50°C), G. amansii biomass (1.0 and 4.0 g·L−1), and agitation mode (agitation or static), were investigated to detect the significance of each factor using a Plackett–Burman design. The analysis of variance for the Ni2+ biosorption percentage indicated that three independent variables (contact time, temperature, and agitation–static mode) exhibited a high level of significance in the Ni2+ biosorption process. Twenty experiments were conducted containing six axial, eight factorial, and six replicates points at center points. The resulting face-centered central composite design analysis data for the biosorption of Ni2+ exhibited a very large variation in the removal percentage of Ni2+, which ranged from 29.73 to 100.00%. The maximum Ni2+ biosorption percentage was achieved in the 16th run with an experimental percentage quantified as 100.00% under the experimental conditions of 3 h of incubation time and 45°C with 100 rpm for agitation speed.