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Background: Survivin, smallest Inhibitor of Apoptosis Protein (IAP) mediates apoptosis pathway and cell cycle is tumor specific and therapeutic target for cancer research. Breast cancer is a dreadful disease with greater mortality rate, commonly in women globally. Aim: This study was undertaken to investigate the effect of indigenous Plant drugs by in-silico determination of antagonist for Survivin protein that was up regulated in breast cancer. Materials and Methods: The 3D structure of Survivin protein was retrieved from PDB (Protein Data Bank). Plant compounds were retrieved from NPACT (Naturally occurring Plant based Anti-Cancerous Target) database and converted into 3D- PDB format using Open babel software. Based on the active sites we have screened the essential plant compound as an effective binder against modelled protein using iGEMDock 2.1. Results and conclusion: The lead compound of plant as well as plant protein molecule would be scaled out on the basis of binding efficiency. Remangilones A showed greater interaction effect with Total energy (-121.465kcal mol-1). However, further in-vivo investigation is essential for the confirmation of drug efficacy and bio-compatibility.

Medicinal plant extracts are known to possess breast cancer antidote. The present investigation is focused on anticancer efficacy of various parts of Annona squamosa. The organic (ethanol) extracts from various parts of Annona squamosa were prepared using soxhlet apparatus and tested for in vitro anticancer efficacy on Breast cancer cell line MCF-7 by MTT (3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. The results obtained from MTT assay showed that the inhibitory concentration values of bark, peel and seed were found to be approximately 20, 30 and 10 μg/ml, respectively The ethanolic seed extract had high anticancer activity with IC50 value of 10 ug/ml, reveals that A. squamosa inhibits the proliferation of MCF-7 by inducing apoptosis. The plant investigated has anti-cancer activity; hence further studies should be carried out for the isolation of the lead molecules from the parts of the plant to treat the breast cancer.

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