We present evidence for the coexistence and coevolution of antibiotic resistance and biosynthesis genes in soil bacteria. The distribution of the streptomycin (strA) and viomycin (vph) resistance genes was examined in Streptomyces isolates. strA and vph were found either within a biosynthetic gene cluster or independently. Streptomyces griseus strains possessing the streptomycin cluster formed part of a clonal complex. All S. griseus strains possessing solely strA belonged to two clades; both were closely related to the streptomycin producers. Other more distantly related S. griseus strains did not contain strA. S. griseus strains with only vph also formed two clades, but they were more distantly related to the producers and to one another. The expression of the strA gene was constitutive in a resistance-only strain whereas streptomycin producers showed peak strA expression in late log phase that correlates with the switch on of streptomycin biosynthesis. While there is evidence that antibiotics have diverse roles in nature, our data clearly support the coevolution of resistance in the presence of antibiotic biosynthetic capability within closely related soil dwelling bacteria. This reinforces the view that, for some antibiotics at least, the primary role is one of antibiosis during competition in soil for resources.

Streptomycin resistant streptomycetes were isolated from four diverse sites. Two sites in Germany were sampled and included an agricultural experimental plot of apple trees which had a history of streptomycin application (AR) and a control site without antibiotic application (CR). Two sites in the UK were sampled; a pastureland site which had sewage injection (DW) and an agricultural site (CW). The actinomycete counts indicated a high proportion of streptomycin resistant isolates in the CW soil. Streptomycetes were identified by partial sequencing of the 16S rDNA. PCR product of the hypervariable gamma region of 16S rDNA allowed analysis by denaturing gradient gel electrophoresis to assess the diversity within the isolates. The streptomycin and sewage sludge treated sites showed decreased diversity within streptomycete populations. Isolates were screened for the streptomycin resistance gene, strA, and flanking biosynthesis gene, strB1. Distribution of these genes indicated the prevalence of str genes in the streptomycin treated soil. Evidence of horizontal gene transfer was recorded in isolates identified as Streptomyces platensis recovered from CR and AR sites which had acquired a streptomycin resistance gene homologous to that found in Streptomyces griseus. Members of the latter species were the most abundant streptomycin resistant streptomycetes isolated from all soils.