A sensitive and validated UV method have been developed for the simultaneous estimation of Ofloxacin (OFL) and Tinidazole (TNZ) in bulk and pharmaceutical dosage form, without prior separation, by three different techniques (Simultaneous equation, Absorbance ratio method and Dual wavelength method). The work was carried out on Shimadzu electron UV1800 double beam UV-Visible spectrophotometer. The absorption spectra of reference and test solutions were carried out in 1 cm matched quartz cell over the range of 200-400 nm. The first method is the application of simultaneous equation, where the linearity ranges for Ofloxacin and Tinidazole were 2-10 μg/ml and 5-15 μg/ml respectively. The second method is the dual wavelength method, where the linearity ranges for Ofloxacin and Tinidazole were 2-10 μg/ml and 5-15 μg/ml respectively. The third method is the determination of ratio of absorbance at 294.6 nm, the maximum absorption of Tinidazole and isobestic wavelength 285.6 nm, the linearity ranges for Ofloxacin and Tinidazole were 2-10 μg/ml and 5-15 μg/ml respectively. The results of the analysis have been validated statistically and by recovery studies. The proposed procedures are rapid, simple, require no preliminary separation steps and can be used for routine analysis of both drugs in quality control laboratories.

Synthetic dyes were frequently utilized in the formulation of marketable lipsticks. However, the practice of synthetic dye has been associated with many health problems. Hence, natural colorant might tender a promising solution to this problem. The Anthocynin which are found in Hibiscus have drawn attention as a potential source of natural colorant to synthetic dye. However, the use of natural colorant in available is limited due to its low stability towards temperature, oxygen, pH, phyoto and colour deterioration across storage

Objectives: The objective of the present study was to synthesis a lipstick with natural color pigment - anthocyanin which extracted from Rosa 'Mister Lincoln' and studies its color stability during storage period. Materials and Methods: Different organic ingredients such as beeswax, Shreeji Wax, vanilla essence, castor oil, olive oil, lemon juice and Anthocyanin extracted from Rosa 'Mister Lincoln' was used for the formulation of herbal lipstick. Results: The Physico-chemical properties of the synthesized lipstick such as spreadability, skin irritation test, and breaking point, surface anomalies, melting point, and perfume stability, homogeneity and color uniformity were determined and compared with commercial lipsticks. The stability of the synthesized lipstick found to be stable under dark condition while color loss was greater for lipstick in light condition. Conclusion: Due to the low pigmentation from method, Anthocyanin is suggested incorporate in to lip balm application. This study has proven that Anthocyanin could replace synthetic dye in cosmetics industry for lip balm application.

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