Gold nanostars (AuNSs) are synthesized by seed mediated growth method. The synthesized AuNSs solution is stable and shows a localized surface plasmon resonance (LSPR) band in the visible range, which is confirmed by UV-visible spectroscopy. Further, the as-synthesized AuNSs were functionalized with melamine and used as a sensor for the colorimetric detection of uric acid (UA). The detection mechanism could be assessed various analytical techniques such as UV-visible spectroscopy, Fourier transforms infrared (FT-IR) spectroscopy, dynamic light scattering (DLS), zeta potential, field emission scanning electron microscopy (FE-SEM) and transmission electron microscopic techniques, respectively. This methods exhibited a good linear regression between the absorption ratio of LSPR band of melamine-AuNSs and the concentration of UA (0 – 120 µM), with the detection limit of 8.50 nM. As a result, UA was quantitatively detected in biofluids by melamine-AuNSs as a colorimetric sensor, revealing melamine-AuNSs-based colorimetric approach could be used as a simple platform for UA assay in biofluids.