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BackgroundVarious natural compounds have an aromatic heterocyclic compound “Indole” consists of bicyclic structure as a parent constituent in their composition that is used as an important precursor in many pharmaceutical industries and is widely accepted as a substance of fragrance to many novel compositions. In present study some synthetic indole derivatives are used to check their biological potential by performing anti-inflammatory, analgesic and anti-pyretic activity in albino mice.MethodsAlbino mice of either sex of reproductive age were used for each study and number of animals for each group in respective studies was kept 5. In anti-inflammatory activity, the Negative Control (NC) and Positive Control (PC) group animals were treated with normal saline and 10mg/kg of indomethacin respectively. The treated groups received the twenty four different synthetic chemicals, after 30 minutes of sub cutaneous injection of carrageenan. In analgesic activity, hot-plate method is used and for each group the latency period was recorded at zero moment of the provision of required dose and after 30, 60, 90, 120 and 180 minutes. In anti-pyretic activity, Pyrexia was induced by using Brewer's yeast method. Before any treatment and then after duration of 18 hours, the rectal temperatures were recorded. The animals, which showed the abnormally high rectal temperature, were selected for the further chemical investigation. SPSS.20 software is used for statistical analysis.ResultsThis study helped to screen out the most potent indole derivative 3a-II and 4a-II from the 24 synthetic indole derivatives which demonstrated the best anti-inflammatory, analgesic and anti-pyretic potential as compared to remaining ones. ConclusionThe above findings indicate that only two chemicals 3a-II (N-(4´-Chlorophenyl) (4,6-dimethoxy-2,3-diphenyl-1H-indol-7-yl)methanimine) and 4a-II (N-(4´-Methylphenyl) (4,6-dimethoxy-2,3-diphenyl-1H-indol-7-yl)methanimine) have given the desired effects like anti-inflammatory, analgesic and anti-pyretic.

A large number of new synthetic compounds are synthesized in the field of heterocyclic chemistry having a variety of biological potentials. In the present study, some synthetic indole derivatives are used to check anti-inflammatory, analgesic, antipyretic and gastroprotective activity in albino mice. Albino mice of either sex of reproductive age were used for each study (n = 5). In anti-inflammatory activity, the negative control (NC) and positive control group animals were treated with normal saline and 10 mg/kg of indomethacin respectively. The treated groups received the twenty four different synthetic chemicals, after 30 min of sub cutaneous injection of carrageenan. In analgesic activity, hot-plate method is used and for each group the latency period was recorded at zero moment of the provision of required dose and after 30, 60, 90, 120 and 180 min. In anti-pyretic activity, Pyrexia was induced by using Brewer's yeast method. Before any treatment and then after duration of 18 h, the rectal temperatures were recorded. Among all the chemicals, only those chemicals which show any potential related to above mentioned activities were selected for gastroprotective activity. The gastroprotective activity was performed to check the gastric ulcers by using 300 mg/kg of single oral dose of indomethacin to animals of all groups except NC group. This study helped to screen out the most potent indole derivatives 3a-II and 4a-II from the 24 synthetic indole derivatives which demonstrated the best biological potential (anti-inflammatory, analgesic, antipyretic, and gastroprotection) as compared to the remaining ones. The micrometric and biochemical results also support the histological findings. Out of the twenty-four novel indole amines tested, 3a-II and 4a-II have shown the effective pharmacological capacity and additionally have not shown any overt and systemic toxicity. Thus these two indole amines need further in-depth pharmacokinetic and pharmacodynamics studies before they are recommended for any pre-clinical trial.