This study was designed to induction of testicular degeneration syndrome (TDS) in male rats by cadmium chloride and identified the best concentration of induction for this syndrome. The effective dose of cadmium chloride (CdCl₂)  was determined by used fifteen males rats and where divided into three equal groups treated with CdCl₂ in a concentration (1, 2 and 3)mg/kg B.W. I.P. one /week for two weeks. Later the experimental animals was scarified and testis was took for measuring the antioxidant parameters via evaluated of glutathione GSH,  malondialdehyde MDA, catalse, albumin, fructose concentrations and aminotransferase activity (ALT and AST). According to this result the lowest observed adverse effective dose (LOAEL) of cadmium chloride that induced testicular degeneration in male rats was found to be 1mg/kg B.W. I.p. one /week for two weeks.