The aim of this work was to study the effect of different NPK rates (0.00, 22.4, 44.8 and 67.2 Kg/fed) and different Mycorrhizal fungi concentrations (0.00, 28, 56 and 84 g/fed) and their interactions on growth and chemical content of dry leaves (nitrogen, phosphorus, potassium), and fresh leaves content of chlorophyll A and B as well as dry flower content of oil of chamomile (Matricaria chamomilla L.) under sandy soil conditions. A Split plot design was used NPK rates were randomly arranged in main plots and the mycorrhizal fungi concentrations were randomly distributed in the sub plots, Drip irrigation was used. Distance between lines was 50 cm and between plants in the same row was 30 cm (28000 plant per fed). The obtained results showed that the highest values of vegetative growth (plant height, stem diameter, number of branches, fresh and dry weight of herb, root length, and root fresh and dry weight), flower traits (number of folwer/plant, flower fresh and dry weight, and flower diameter), chemical constituents (N, P, K), chlorophyll content (a & b), and essential oil percentage were achieved when chamomile plants treated with 67.2 kg NPK/fed or 84g/fed mycorrhizal fungi. The interaction results indicated that the highest values of all previous traits were recorded with plants fertilized with 67.2 kg NPK/fed and inoculated with mycorrhizal fungi at 84 g/fed.