Objective: Eating disorders (EDs) and depression are impacting youth at alarming rates, yet most adolescents do not access treatment. Single-session interventions (SSIs) can reach youth in need. This pilot examines the acceptability and short-term utility of an SSI designed to help adolescents adopt a body neutrality mindset. Method: Pre- to post-intervention data was collected, and within-group effect sizes and 95% confidence intervals were computed, to evaluate the immediate effects of the SSI on hopelessness, functionality appreciation, and body dissatisfaction. Patterns of use, demographics, program feedback, and responses from within the SSI were also collected. Results: The SSI and all questionnaires were completed by 75 adolescents (ages 13-17, 74.70% White/Caucasian, 48.00% woman/girl) who reported elevated body image and mood problems. Analyses detected significant pre-post improvements in hopelessness (dav = 0.60, 95% CI 0.35, 0.84; dz = 0.77, 95% CI 0.51, 1.02), functionality appreciation (dav = 0.72, 95% CI 0.46, 0.97; dz = 0.94, 95% CI 0.67, 1.21), and body dissatisfaction (dav = 0.61, 95% CI 0.36, 0.86; dz = 0.76, 95% CI 0.50, 1.02). The SSI was rated as highly acceptable, with a mean overall score of 4.34/5 (SD = 0.54). Qualitative feedback suggested adolescents' endorsement of body neutrality as a personally-relevant, helpful target for intervention. Discussion: This evaluation supports the acceptability and preliminary effectiveness of a body neutrality-focused SSI for adolescents with body image and mood concerns.

Data Availability Statement:The data and code that support the findings of this evaluation, excluding identifying information, are openly available at https://osf.io/w82bf/.

Understanding the gut microbiota characteristics of endangered species such as the Eurasian otter (Lutra lutra), especially in their early stages of life, could be essential for improving their management and ex situ conservation strategies. Here, we analyzed the gut microbiota diversity, composition, and function of captive Eurasian otters at different ages using high-throughput 16S rRNA gene sequencing. We found that: (1) Clostridiaceae was abundant in all age stages; (2) Lactococcus in cubs is thought to predominate for digesting milk; (3) bacteria associated with amino acid metabolism increase with age, while bacteria associated with carbohydrate metabolism decrease with age, which is likely due to decrease in dietary carbohydrate content (e.g., milk) and increase in dietary protein contents (e.g., fishes) with age; and (4) fish-related bacteria were detected in feces of healthy adults and juveniles. Overall, the gut microbiota of captive Eurasian otters was taxonomically and functionally different by age, which is thought to be attributed to the difference in the diet in their life stages. This study provided baseline information regarding the gut microbiota of Eurasian otters for the first time and contributes to improvement in their management in captivity.

The SAINOME-plate consists of a 384-well plate and a cover that contains a cutter, which can cut a polyacrylamide gel into approximately 4.5-mm square pieces following electrophoresis. In this study, we applied SDS-PAGE and the SAINOME-plate to fractionation of protein mixtures from cell extracts or serum for proteomic approaches. Compared with gel-fractionation using a cutter or a scalpel, SAINOME-plate gel-fractionation is simpler and higherthroughput. In terms of reproducibility of proteomic profiling, SAINOME-plate gel-fractionation was comparable to scalpel gel-fractionation. Additionally, human keratin contamination was lower with the SAINOME-plate than with a scalpel. In serum protein fractionation, the number of proteins identified increased approximately 2-fold and 3.7-fold relative to non-fractionation when the gel was divided into 8 and 96 fractions, respectively. The results demonstrate that the SAINOME-plate gel-fractionation will be a useful method in mass spectrometry-based proteomics.

On March 11, 2011, the 2011 off the Pacific coast of Tohoku Earthquake occurred. After the earthquake, at coast in Iwate prefecture, same as other coastal area, fishermen evacuated to offshore area to protect themselves and their boats. We conducted survey to investigate the evacuation of fisheries in Iwate prefecture and consider whether they can get enough information about tsunami. The results of interview are (1) Evacuation ratio of boats is proportional to the boat size. (2) The depth of the areas boats evacuated to have a variation, and some of them are shallower than 50m. (3) Fishermen on small boats use cell-phone not radio communication system. However at 2011.3.11 cell phone lines were dead or jammed. (4) As boats smaller than about 3tonnage in Fudai and Tanohata, can't get the information of tsunami warning in some area, there is risk to fail to escape from tsunami.