Several new visnaginone ethers were prepared and their thiosemicarbazone derivatives are synthesized. The behavior of the thiosemicarbazones in methanolic sodium methoxide and hydrochloric acid is discussed. Structures were established on the bases of elemental and spectral data studies. Some of the thiosemicarbazones were tested for their antimicrobial activity. The structures of the synthesized derivatives (5a-g,6a-g) were confirmed by means of IR, 1H NMR, MS and elemental analyses. The synthesized derivatives (5a-g,6a-g) wear subjected to the Microbiology Division, Microanalytical Center, Cairo University, (5a-g,6a-g) showed a variable degree of antimicrobial activity.