Arsenic (As) contaminated water is used in South Asian countries to irrigate food crops, but the subsequent uptake of As by vegetables and associated human health risk is poorly understood. We used a pot trial to determine the As uptake of four vegetable species (carrot, radish, spinach and tomato) with As irrigation levels ranging from 50 to 1000 μg L(-1) and two irrigation techniques, non-flooded (70% field capacity for all studied vegetables), and flooded (110% field capacity initially followed by aerobic till next irrigation) for carrot and spinach only. Only the 1000 μg As L(-1) treatment showed a significant increase of As concentration in the vegetables over all other treatments (P < 0.05). The distribution of As in vegetable tissues was species dependent; As was mainly found in the roots of tomato and spinach, but accumulated in the leaves and skin of root crops. There was a higher concentration of As in the vegetables grown under flood irrigation relative to non-flood irrigation. The trend of As bioaccumulation was spinach > tomato > radish > carrot. The As concentration in spinach leaves exceeded the Chinese maximum permissible concentration for inorganic As (0.05 μg g(-1) fresh weight) by a factor of 1.6 to 6.4 times. No other vegetables recorded an As concentration that exceeded this threshold. The USEPA parameters hazard quotient and cancer risk were calculated for adults and adolescents. A hazard quotient value greater than 1 and a cancer risk value above the highest target value of 10(-4) confirms potential risk to humans from ingestion of spinach leaves. In our study, spinach presents a direct risk to human health where flood irrigated with water containing an arsenic concentration greater than 50 μg As L(-1).

Living plants growing on mine waste were assisted to accumulate gold to a concentration greater than 3500 mg kg–1 (0.35%) dry weight. Where Au was the only high-density metal present in the soil at high concentration, discrete biogenic nanoparticles of the metal were observed in preparations of shoot and leaf biomass. Where an equal concentration of silver was additionally present, a nanoalloy of Au and Ag was observed. An equal concentration of copper in the soil had no chemical affect on the Au and/or Ag structures synthesized and did not alloy with Au and/or Ag. However, both Cu and Ag reduced the size of Au nanoparticles, and the presence of Cu and/or Ag also limited the extent to which Au was reduced in plant tissues to Au(0). Ash prepared from Au-only plant material had a visible reflectance spectrum indicative of a Au surface plasmon resonance that was not apparent for the Au/Ag biomass. The Au concentration of the plant ash (3.2–7.2%) was of the same order of magnitude as Au reference catalysts. Therefore, biogenic synthesis is proposed as a valid technique to create high concentration Au structures in a carbon matrix. Our data suggest that such biogenic nanoparticles should be tested in a range of environmental and industrial reactions.

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The application of an acidic biochar can improve plant growth and soil properties in saline conditions. In this study, we investigated the effect of acidic biochar on plant growth and nutrients contents in saline soil. Seven treatments were arranged in a complete randomized design, including control (CK), 0, 30, and 45 g biochar added to a soil having 1% and 1.5% salts; these treatments were termed as B0S1, B30S1, B45S1 and B0S1.5, B30S1.5, B45S1.5 respectively. Experimental results showed that the plant height, leaves plant−1, leaf area, and shoot fresh and dry biomass, and root fresh and dry biomass were increased for the B45S1.5, respectively. Similarly, the highest total nitrogen (TN), total phosphorus (TP), total potassium (TK), and total sodium (Na) concentration in maize shoot were observed for B30S1, B0S1.5, CK, and B0S1.5, respectively. The highest concentrations of TN, TP, TK, and Na in root were obtained with the treatments B0S1, B0S1, B45S1, and B0S1, respectively. Soil pH, and EC decreased and nutrients concentration improved by the addition of acidic biochar. We conclude that the use of acidic biochar can be a potential source for the improvement of maize plant growth as well as mitigate the adverse effect of salt stress.

Biochar has extensively been used for multiple purposes in agriculture, including improving soil microbial biomass. The current study aimed to investigate the effect of acidic biochar on maize seedlings’ rhizosphere bacterial abundance under salinity. There were seven treatments and three replicates in a controlled greenhouse coded as B0S1, B1S1, and B2S1 and B0S2, B1S2, and B2S2. CK is control (free of biochar and salt); B0, B1, and B2 are 0, 15, and 30 g biochar (kg soil)–1; and S1 and S2 are 2.5 and 5 g salt pot–1 that were amended, respectively. After harvesting the maize seedlings, the soil samples were collected and analyzed for soil microbial biomass, bacterial abundance, and diversity. The results revealed that relative abundance of Proteobacteria, Actinobacteria, and Chloroflexi increased on phylum level, whereas Actinomarinales, Alphaproteobacteria, and Streptomyces enhanced on genus level, respectively, in B2S1 and B2S2, when compared with CK and non-biochar amended soil under saline conditions. The relative abundance of Actinomarinales was positively correlated with total potassium (TK) and Gematimonadetes negatively correlated with total phosphorus (TP). Biochar addition slightly altered the Ace1, Chao1, and alpha diversity. Principal component analysis corresponded to the changes in soil bacterial community that were closely associated with biochar when compared with CK and salt-treated soils. In conclusion, acidic biochar showed an improved soil microbial community under salinity.