Autism is associated with gastrointestinal dysfunction and gut microbiota dysbiosis, including an overall increase in Clostridium. Modulation of the gut microbiota is suggested to improve autistic symptoms. In this study, we explored the implementation of two different interventions that target the microbiota in a rodent model of autism and their effects on social behavior: the levels of different fecal Clostridium spp., and hippocampal transcript levels. Autism was induced in young Sprague Dawley male rats using oral gavage of propionic acid (PPA) for three days, while controls received saline. PPA-treated animals were divided to receive either saline, fecal transplant from healthy donor rats, or Bifidobacterium for 22 days, while controls continued to receive saline. We found that PPA attenuated social interaction in animals, which was rescued by the two interventions. PPA-treated animals had a significantly increased abundance of fecal C. perfringens with a concomitant decrease in Clostridium cluster IV, and exhibited high hippocampal Bdnf expression compared to controls. Fecal microbiota transplantation or Bifidobacterium treatment restored the balance of fecal Clostridium spp. and normalized the level of Bdnf expression. These findings highlight the involvement of the gut–brain axis in the etiology of autism and propose possible interventions in a preclinical model of autism.
BackgroundTo evaluate the knowledge and attitude level of recent dental graduates in Saudi Arabia toward stem cells and their potential therapeutic applications.MethodsIn this cross-sectional study, a questionnaire was distributed among 606 recent graduates from 14 dental schools in Saudi Arabia. Fifteen statements were formulated to address the knowledge component with “yes” or “no” or “I do not know” answer and 10 statements were designed to assess the attitude level with Likert 5-point response scale. The level of knowledge for each respondent was designated as poor if the score value is (0–5), moderate (6–10), and high (11–15). To evaluate the attitude level for each respondent, a score of (1) was assigned for extremely negative answer and (5) for extremely positive answer for each statement. Based on the total score, attitude level was considered poor when the score value is (10–29), moderately positive (30–39), good (40–44), or excellent (45–50).ResultsSignificant difference was found in the knowledge score between males and females (p = 0.004). Both males and females showed poor knowledge with a mean score of 5.06 ± 2.5 and 5.63 ± 2.4, respectively. There was an overall moderately positive attitude of participants toward stem cells and their therapeutic applications with a mean value of 33.15 ± 5.92 with no significant difference between males and females (p = 0.53). A significant positive linear correlation was detected between knowledge and attitude for both males (r = 0.323, p = 0.00) and females (r = 0.392, p = 0.00).ConclusionUpdating dental curricula with topics on stem cell research and potential future applications in dentistry is demanded.
Autism spectrum disorders (ASD) consist of a range of neurodevelopmental conditions accompanied by dysbiosis of gut microbiota. Therefore, a number of microbiota manipulation strategies were developed to restore their balance. However, a comprehensive comparison of the various methods on gut microbiota is still lacking. Here, we evaluated the effect of Bifidobacterium (BF) treatment and fecal microbiota transplantation (FT) on gut microbiota in a propionic acid (PPA) rat model of autism using 16S rRNA sequencing. Following PPA treatment, gut microbiota showed depletion of Bacteroidia and Akkermansia accompanied by a concomitant increase of Streptococcus, Lachnospiraceae, and Paraeggerthella. The dysbiosis was predicted to cause increased levels of porphyrin metabolism and impairments of acyl-CoA thioesterase and ubiquinone biosynthesis. On the contrary, BF and FT treatments resulted in a distinct increase of Clostridium, Bifidobacterium, Marvinbryantia, Butyricicoccus, and Dorea. The taxa in BF group positively correlated with vitamin B12 and flagella biosynthesis, while FT mainly enriched flagella biosynthesis. In contrast, BF and FT treatments negatively correlated with succinate biosynthesis, pyruvate metabolism, nitrogen metabolism, beta-Lactam resistance, and peptidoglycan biosynthesis. Therefore, the present study demonstrated that BF and FT treatments restored the PPA-induced dysbiosis in a treatment-specific manner.
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