Objective: To determine whether p53 expression and DNA ploidy were related to traditional prognostic indicators in patients with ovarian cancer. Methods: Tumour material (n = 188) was analysed regarding flow cytometric DNA ploidy and the immunohistochemical p53 expression. Pearson correlation, Fisher's exact test, Cox's regression analysis and Kaplan-Meier survival tests were used, as appropriate. Results: p53 accumulation and aneuploidy were more frequent in patients with serous histology, FIGO advanced stage, who were poorly graded, and had positive peritoneal cytology and tumour recurrence. Patient's age, stage, grade, tumour residue, positive peritoneal cytology, and overall survival rate correlated with p53 accumulation and DNA ploidy. No association of histological subtype with p53 and DNA ploidy was observed. p53 was also strongly related to DNA ploidy. p53 and DNA ploidy were found to be important determinants of recurrence in univariate analyses. Of the factors analysed, p53 was the significantly stronger independent predictor factor for tumour recurrence. Utilizing survival as the endpoint for multivariate analysis, age, grade, tumour residue, and DNA ploidy were independent prognostic indicators. Conclusions: Zusammenfassung Fragestellung: Es wurde das Verhältnis der p53-Akkumulation und DNA-Ploidie zu herkömmlichen prognostischen Indikatoren bei Patienten mit Ovarialkarzinom untersucht. Material und Methodik: Das Tumormaterial (n = 188) wurde in Bezug auf die durchflusszytometrische DNA-Ploidie und auf die immunohistochemische Akkumulation des p53-Proteins analysiert. Dementsprechend wurden die Pearson Korrelation, Fisher's Exact Test, Cox's Regressionsanalyse und die Kaplan-Meier Überlebensmethode verwendet. Ergebnisse: Die p53-Akkumulation und Aneuploidie waren häufiger bei Patienten mit seröser Histologie, einem fortgeschrittenen FIGO-Stadium mit ungünstigem Grad, und die eine positive peritoneale Zytologie und ein Wiederauftreten des Tumors aufwiesen. Das Alter der Patienten, Stadium, Grad, Resttumor, positive peritoneale Zytologie und Gesamtüberlebensrate korrelierten mit der DNA-Ploidie und der p53-Akkumulation. Es wurde keine signifikante Assoziation des histologischen Subtyps mit p53 und der DNA-Ploidie beobachtet. p53 und DNA-Ploidie waren stark korreliert. Es stellte sich in Univarianzanalyse heraus, dass p53 und DNA-Ploidie wichtige Bestimmungsfaktoren des Wiederauftretens waren. Von den analy

We have shown that false-positivity of mediastinal lymph nodes had yielded worse survival in surgically staged or resected NSCLC patients staged with PET/CT. This result may help to allocate patients with potentially poor prognosis for considered additional therapy.

The maximum standardized uptake value (SUV(max)) varies among positron emission tomography-integrated computed tomography (PET/CT) centers in the staging of non-small cell lung cancer. We evaluated the ratio of the optimum SUV(max) cut-off for the lymph nodes to the median SUV(max) of the primary tumor (ratioSUV(max)) to determine SUV(max) variations between PET/CT scanners. The previously described PET predictive ratio (PPR) was also evaluated. PET/CT and mediastinoscopy and/or thoracotomy were performed on 337 consecutive patients between September 2005 and March 2009. Thirty-six patients were excluded from the study. The pathological results were correlated with the PET/CT findings. Histopathological examination was performed on 1136 N2 lymph nodes using 10 different PET/CT centers. The majority of patients (group A: 240) used the same PET/CT scanner at four different centers. Others patients were categorized as group B. The ratioSUV(max) for groups A and B was 0.18 and 0.22, respectively. The same ratio for centers 1, 2, 3 and 4 was 0.2, 0.21, 0.21, and 0.23, respectively. The optimal cut-off value of the PPR to predict mediastinal lymph node pathology for malignancy was 0.49 (likelihood ratio +2.02; sensitivity 70%, specificity 65%). We conclude that the ratioSUV(max) was similar for different scanners. Thus, SUV(max) is a valuable cut-off for comparing-centers.

A sternal wedge osteotomy is more commonly performed in patients with pectus excavatum compared to those with pectus carinatum. All redo and pectus arcuatum cases need a wedge osteotomy for proper correction. Wedge osteotomy is very likely in more aggressive corrections with more rib resections.

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