Latar Belakang. TBC masih merupakan masalah kesehatan di Indonesia. Pemerintah telah menerapkan DOTS untuk menurunkan angka kesakitan dan kematian akibat TBC, namun angka tersebut masih belum mencapai target. Selama pandemi, DOTS tetap diselenggarakan dengan adanya penyesuaian pengelolaan input dan process.Tujuan. Tujuan dari penelitian ini yakni mengetahui gambaran pelaksanaan program DOTS selama pandemi di wilayah Puskesmas Depok Jaya.Metode. Penelitian ini menggunakan pendekatan kualitatif desain studi kasus. Teknik pengumpulan data wawancara mendalam kepada informan utama, yakni Penanggung Jawab DOTS, Dokter Penanggung Jawab DOTS, Ketua Kader dan 2 Pengawas Menelan Obat, sedangkan informan pendukung, yakni 4 Pasien TBC. Peneliti mengambil data dengan wawancara mendalam secara daring melalui Zoom.Hasil. Beberapa kegiatan utama di Puskesmas selama pandemi mengalami penurunan jumlah kegiatan, diantaranya investigasi kontak, skrining, serta pelatihan. Selain itu terdapat beberapa masalah di pelaksanaan DOTS yang terjadi selama pandemi, yaitu masyarakat yang kurang terbuka, memiliki mobilitas yang tinggi, sehingga petugas puskesmas dan kader kesehatan seringkali kesulitan dalam melakukan pemantauan terkait dengan investigasi kontak dan pengobatan pasien TBC.Kesimpulan. Gambaran pelaksanaan program DOTS selama pandemi di wilayah Puskesmas Depok Jaya belum maksimal berjalan dikarenakan mengalami penurunan jumlah kegiatan.     ABSTRACT    Background. TB is a problem in Indonesia. The government has implemented DOTS to reduce morbidity and mortality but hasn’t yet reached the target. During the pandemic, DOTS was being held with adjustments to input and process management.Purpose. Find out the description of the implementation of DOTS during a pandemic in the Puskesmas Depok Jaya area. Methods. This study used a qualitative case study design. Data collection techniques were in-depth interviews with the main informants, the PIC of DOTS, the doctor in charge of DOTS, the head of the cadre and 2 medication supervisors, the supporting informants were 4 tuberculosis patients. Researchers collected data by in-depth interviews online via Zoom. Results. Several main activities at the Puskesmas during the pandemic experienced a decrease in the number of activities, including contact investigations, screening, and training. There were several problems that occurred during the pandemic, the community was less open, had high mobility, so Puskesmas officers and health cadres had difficulties in carrying out monitoring related to contact investigations and treatment of TB patients. Conclusion. DOTS hasn’t run optimally due to a decrease in the number of activities.