Two new Cyrtodactylus species are described from Houaphan and Luang Prabang provinces in Laos based on morphological and molecular data. Cyrtodactylus houaphanensis sp. nov. differs from all other Cyrtodactylus in the C. wayakonei species group by at least 3.3 % genetic divergence in the COI gene and can be diagnosed in morphology as follows: SVL 75.8 mm; supralabials 9 or 10; infralabials 8 or 9; ventral scales 35; dorsal tubercles in 20 rows at midbody; precloacal pores 6 in the male; femoral pores absent; subcaudals enlarged; five irregular, brown bands between limb insertions. The new species morphologically resembles C. chauquangensis and revealed to be a sister taxon to C. puhuensis according to our genetic analyses, from which it mainly differs in the absence of enlarged femoral scales. Cyrtodactylus ngoiensis sp. nov. differs from other closely related congeners by at least 11.6 % genetic divergence in the COI gene and can be diagnosed in morphology as follows: maximum SVL 95.3 mm; supralabials 6–9; infralabials 8–11; ventral scales 38–43; dorsal tubercles in 15–21 rows at midbody; enlarged femoral scales present; precloacal pores 7 in the male, 7 pitted scales in females; femoral pores 14 in the male, absent in females; five bright yellowish transverse dorsal bands; subcaudals enlarged. The new species is shown to be a member of the C. wayakonei species group, but morphologically resembles C. dumnuii from Thailand. According to our phylogenetic analyses, it is a basal taxon to a clade comprising C. spelaeus, C. chauquangensis, C. vilaphongi, C. cucphuongensis, C. puhuensis, C. houaphanensis, C. otai and C. bobrovi. Based on a new record of C. bansocensis from central Laos, which represents the first recorded female we provide a redescription of C. bansocensis including expanded diagnosis. In addition, an updated identification key for the Cyrtodactylus known from Laos is provided.

A new species of the genus Cyrtodactylus is described from Dien Bien Province, northwestern Vietnam based on morphological and molecular data. Cyrtodactylus ngati sp. nov. can be distinguished from remaining congeners by the following combination of characters: maximum SVL 69.3 mm; dorsal pattern consisting of six dark irregular transverse bands between limb insertions; inter-supranasals one; dorsal tubercles present on occiput, body, hind limbs and on first half of tail; 17–22 irregular dorsal tubercle rows at midbody; lateral folds clearly defined, with interspersed tubercles; 32–38 ventral scales between ventrolateral folds; 13 precloacal pores separated by a diastema of 5/5 poreless scales from a series of 7/7 femoral pores in enlarged femoral scales; precloacal and femoral pores absent in females; 1–3 postcloacal tubercles on each side; transversely enlarged median subcaudal scales absent. In the molecular analyses, the new species is shown to be the sister taxon to C. interdigitalis from Thailand. This is the 47th species of the genus Cyrtodactylus and the first member of the C. brevipalmatus species group recorded from Vietnam.

A new species of the genus Cyrtodactylus from Vientiane Province, northern Laos is described based on morphological and molecular data. Cyrtodactylus muangfuangensis sp. nov. can be distinguished from remaining congeners by the following combination of characters: maximum SVL 83.9 mm; dorsal pattern consisting of dark nuchal loop, nape band and five dark transversal bands between limb insertions; intersupranasals two; dorsal tubercles present on occiput, body, hind limbs and tail base; 15 or 16 irregular dorsal tubercle rows at midbody; lateral folds clearly defined, without interspersed tubercles; 31–37 ventral scales between ventrolateral folds; six precloacal pores and 15 femoral pores in males, which are interrupted by six to eight poreless scales; six precloacal pitted scales plus in total 10–15 pitted femoral scales in females, which are separated by six to eight poreless scales; enlarged precloacal and femoral scales present; two or three postcloacal tubercles; median subcaudal scales transversely enlarged. In molecular analyses, the new species is strongly supported as a member of the Cyrtodactylus phongnhakebangensis species group, and weakly corroborated as a sister taxon to C. pageli. Pairwise genetic comparison shows that it is at least 18% divergent from other congeners in the species group based on a fragment of the mitochondrial cytochrome C oxidase subunit I gene.